PXD045808-2

PXD045808 is an original dataset announced via ProteomeXchange.

Title	Regulation of the DLC3 tumor suppressor by a novel phosphoswitch
Description	Deleted in Liver Cancer 3 (DLC3) is a Rho GTPase-activating protein (RhoGAP) that plays a crucial role in maintaining adherens junction integrity and coordinating polarized vesicle transport by modulating Rho activity at the plasma membrane and endomembranes. By employing bioinformatical sequence analysis, in vitro experiments, and in cellulo assays a polybasic region (PBR) responsible for mediating the association of DLC3 with cellular membranes was identified. It was shown that the DLC3 PBR contains two novel phosphorylation sites that can alter the local electrostatic character of the PBR and its ability to interact with negatively charged membranes. Accordingly, upon introduction of phosphomimetic mutations into the DLC3 PBR, membrane association of DLC3 was impaired. Furthermore, we uncovered a distinct, PBR-dependent localization of DLC3 at the midbody during cell division, where it exerted control over local Rho activity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:43:38.214.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mirita Franz-Wachtel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methylated lysine; phosphorylated residue; methylated arginine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-29 05:32:04	ID requested
1	2024-06-04 01:24:28	announced
⏵ 2	2024-10-22 06:43:38	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: cytokinesis, GTPase activating protein,Rho signaling, polybasic region, membrane binding

Monilola A. Olayioye
contact affiliation	University of Stuttgart, Institute of Cell Biology and Immunology, Stuttgart, Germany
contact email	monilola.olayioye@izi.uni-stuttgart.de
lab head
Mirita Franz-Wachtel
contact affiliation	Proteome Center Tuebingen
contact email	mirita.franz@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD045808

PRIDE project URI

• PRIDE
  1. PXD045808
     1. Label: PRIDE project
     2. Name: Regulation of the DLC3 tumor suppressor by a novel phosphoswitch