PXD045808 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Regulation of the DLC3 tumor suppressor by a novel phosphoswitch |
Description | Deleted in Liver Cancer 3 (DLC3) is a Rho GTPase-activating protein (RhoGAP) that plays a crucial role in maintaining adherens junction integrity and coordinating polarized vesicle transport by modulating Rho activity at the plasma membrane and endomembranes. By employing bioinformatical sequence analysis, in vitro experiments, and in cellulo assays a polybasic region (PBR) responsible for mediating the association of DLC3 with cellular membranes was identified. It was shown that the DLC3 PBR contains two novel phosphorylation sites that can alter the local electrostatic character of the PBR and its ability to interact with negatively charged membranes. Accordingly, upon introduction of phosphomimetic mutations into the DLC3 PBR, membrane association of DLC3 was impaired. Furthermore, we uncovered a distinct, PBR-dependent localization of DLC3 at the midbody during cell division, where it exerted control over local Rho activity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-04 |
AnnouncementXML | Submission_2024-06-04_01:24:28.494.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mirita Franz-Wachtel |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | methylated lysine; phosphorylated residue; methylated arginine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-29 05:32:04 | ID requested | |
⏵ 1 | 2024-06-04 01:24:28 | announced | |
2 | 2024-10-22 06:43:38 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: cytokinesis, GTPase activating protein,Rho signaling, polybasic region, membrane binding |
Contact List
Monilola A. Olayioye |
contact affiliation | University of Stuttgart, Institute of Cell Biology and Immunology, Stuttgart, Germany |
contact email | monilola.olayioye@izi.uni-stuttgart.de |
lab head | |
Mirita Franz-Wachtel |
contact affiliation | Proteome Center Tuebingen |
contact email | mirita.franz@uni-tuebingen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045808
- Label: PRIDE project
- Name: Regulation of the DLC3 tumor suppressor by a novel phosphoswitch