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PXD045808-1

PXD045808 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRegulation of the DLC3 tumor suppressor by a novel phosphoswitch
DescriptionDeleted in Liver Cancer 3 (DLC3) is a Rho GTPase-activating protein (RhoGAP) that plays a crucial role in maintaining adherens junction integrity and coordinating polarized vesicle transport by modulating Rho activity at the plasma membrane and endomembranes. By employing bioinformatical sequence analysis, in vitro experiments, and in cellulo assays a polybasic region (PBR) responsible for mediating the association of DLC3 with cellular membranes was identified. It was shown that the DLC3 PBR contains two novel phosphorylation sites that can alter the local electrostatic character of the PBR and its ability to interact with negatively charged membranes. Accordingly, upon introduction of phosphomimetic mutations into the DLC3 PBR, membrane association of DLC3 was impaired. Furthermore, we uncovered a distinct, PBR-dependent localization of DLC3 at the midbody during cell division, where it exerted control over local Rho activity.
HostingRepositoryPRIDE
AnnounceDate2024-06-04
AnnouncementXMLSubmission_2024-06-04_01:24:28.494.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMirita Franz-Wachtel
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmethylated lysine; phosphorylated residue; methylated arginine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Orbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-29 05:32:04ID requested
12024-06-04 01:24:28announced
22024-10-22 06:43:38announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: cytokinesis, GTPase activating protein,Rho signaling, polybasic region, membrane binding
Contact List
Monilola A. Olayioye
contact affiliationUniversity of Stuttgart, Institute of Cell Biology and Immunology, Stuttgart, Germany
contact emailmonilola.olayioye@izi.uni-stuttgart.de
lab head
Mirita Franz-Wachtel
contact affiliationProteome Center Tuebingen
contact emailmirita.franz@uni-tuebingen.de
dataset submitter
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Dataset FTP location
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