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PXD045741-2

PXD045741 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMmoD regulates soluble methane monooxygenase and methanobactin production in Methylosinus trichosporium OB3b
DescriptionMethane oxidation by aerobic methanotrophs is well-known to be strongly regulated by the availability of copper, i.e., the “copper-switch”. That is, there are two forms of the methane monooxygenase: a cytoplasmic or soluble methane monooxygenase (sMMO) and a membrane-bound or particulate methane monooxygenase (pMMO). sMMO is only expressed and active in the absence of copper, while pMMO requires copper. Previous work has also shown that one gene in the operon of the soluble methane monooxygenase – mmoD – also plays a critical role, but its function is still vague. Herein we show that MmoD is not needed for expression of genes in the sMMO gene cluster but is critical for formation of sMMO polypeptides and sMMO activity in Methylosinus trichosporium OB3b, indicating that MmoD plays a key post-transcriptional role in maturation of sMMO. Further, data also show that MmoD controls expression of methanobactin, a unique copper-binding compound used by some methanotrophs for copper collection. Collectively these results provide greater insights into the components of the “copper-switch” and thus provide new strategies to manipulate methanotrophic activity.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:04:22.926.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHenriette Remmer
SpeciesList scientific name: Methylosinus trichosporium OB3b; NCBI TaxID: 595536;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-27 10:57:12ID requested
12023-09-29 10:07:52announced
22024-10-22 06:04:23announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: methanotrophy, methane monooxygenase, Methylosinus trichosporium OB3b ΔmmoD, methanobactin
Contact List
Henriette Remmer
contact affiliationHenriette A. Remmer, Ph.D. Director, Proteomics & Peptide Synthesis Core University of Michigan Michigan Medicine C570C MSRB II SPC 5674
contact emailhremmer@med.umich.edu
lab head
Henriette Remmer
contact affiliationUniversity of Michigan
contact emailhremmer@umich.edu
dataset submitter
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Dataset FTP location
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