PXD045741-1

PXD045741 is an original dataset announced via ProteomeXchange.

Title	MmoD regulates soluble methane monooxygenase and methanobactin production in Methylosinus trichosporium OB3b
Description	Methane oxidation by aerobic methanotrophs is well-known to be strongly regulated by the availability of copper, i.e., the “copper-switch”. That is, there are two forms of the methane monooxygenase: a cytoplasmic or soluble methane monooxygenase (sMMO) and a membrane-bound or particulate methane monooxygenase (pMMO). sMMO is only expressed and active in the absence of copper, while pMMO requires copper. Previous work has also shown that one gene in the operon of the soluble methane monooxygenase – mmoD – also plays a critical role, but its function is still vague. Herein we show that MmoD is not needed for expression of genes in the sMMO gene cluster but is critical for formation of sMMO polypeptides and sMMO activity in Methylosinus trichosporium OB3b, indicating that MmoD plays a key post-transcriptional role in maturation of sMMO. Further, data also show that MmoD controls expression of methanobactin, a unique copper-binding compound used by some methanotrophs for copper collection. Collectively these results provide greater insights into the components of the “copper-switch” and thus provide new strategies to manipulate methanotrophic activity.
HostingRepository	PRIDE
AnnounceDate	2023-09-29
AnnouncementXML	Submission_2023-09-29_10:07:52.603.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HenrietteRemmer
SpeciesList	scientific name: Methylosinus trichosporium OB3b; NCBI TaxID: 595536;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-27 10:57:12	ID requested
⏵ 1	2023-09-29 10:07:52	announced
2	2024-10-22 06:04:23	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: methanotrophy, methane monooxygenase, Methylosinus trichosporium OB3b ΔmmoD, methanobactin

HenrietteRemmer
contact affiliation	Henriette A. Remmer, Ph.D. Director, Proteomics & Peptide Synthesis Core University of Michigan Michigan Medicine C570C MSRB II SPC 5674
contact email	hremmer@med.umich.edu
lab head
HenrietteRemmer
contact affiliation	University of Michigan
contact email	hremmer@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD045741

PRIDE project URI

• PRIDE
  1. PXD045741
     1. Label: PRIDE project
     2. Name: MmoD regulates soluble methane monooxygenase and methanobactin production in Methylosinus trichosporium OB3b