PXD044337 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Inducible degradation-coupled phosphoproteomics identifies PP2A-Rts1 as a novel eisosome regulator |
Description | We developed and validated a workflow in S. cerevisiae combining auxin-inducible protein degradation with LC-MS/MS phosphoproteomics and used it to identify candidate phosphatase substrates during mitosis, when kinase activity is high. Our results revealed that PP2A-Rts1 influences dephosphorylation of numerous proteins in mitosis, including multiple subunits of the plasma membrane-associated eisosome complex. |
HostingRepository | PRIDE |
AnnounceDate | 2023-10-24 |
AnnouncementXML | Submission_2023-10-24_06:30:20.020.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | MarkHall |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-03 16:15:18 | ID requested | |
⏵ 1 | 2023-10-24 06:30:20 | announced | |
2 | 2024-10-22 06:07:17 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Phosphoproteomics, PP2A, Rts1,Phosphatase, auxin inducible degradation |
Contact List
MarkHall |
contact affiliation | Purdue University, Department of Biochemistry |
contact email | mchall@purdue.edu |
lab head | |
MarkHall |
contact affiliation | Purdue University |
contact email | mchall@purdue.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044337
- Label: PRIDE project
- Name: Inducible degradation-coupled phosphoproteomics identifies PP2A-Rts1 as a novel eisosome regulator