PXD043946-2

PXD043946 is an original dataset announced via ProteomeXchange.

Title	Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility
Description	Spermatogenesis is a crucial biological process that enables the production of functional sperm, allowing for successful reproduction. Proper germ cell differentiation and maturation require tight regulation of hormonal signals, cellular signaling pathways, and cell biological processes. The acrosome is a lysosome-related organelle at the anterior of the sperm head that contains enzymes and receptors essential for egg-sperm recognition and fusion. Even though several factors crucial for acrosome biogenesis have been discovered, the precise molecular mechanism of pro-acrosomal vesicle formation and fusion is not known. In this study, we investigated the role of the insulin inhibitory receptor (inceptor) in acrosome formation. Inceptor is a single-pass transmembrane protein with similarities to mannose-6-phosphate receptors (M6PR). Inceptor knockout mice are infertile due to malformations in the acrosome and defects in the nuclear shape of spermatozoa. We show that inceptor is expressed in early spermatids and mainly localizes to vesicles between the Golgi apparatus and acrosome. We propose that inceptor is an important factor in the intracellular transport of trans-Golgi network (TGN)-derived clathrin-coated vesicles delivering acrosomal cargo in maturing spermatids, and its absence results in vesicle-fusion defects, acrosomal malformation, and male infertility. These findings support our hypothesis of inceptor as a universal lysosomal or lysosome-related organelle sorting receptor expressed in several secretory tissues.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:26:32.448.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Bilekova
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-20 03:09:36	ID requested
1	2023-08-28 05:38:01	announced
⏵ 2	2023-11-14 07:26:32	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: vesicle, localization,acrosome, differentiation, spermatogenesis, male fertility

Stefanie Hauck
contact affiliation	Metabolomics and Proteomics Core, Helmholtz Zentrum Muenchen
contact email	hauck@helmholtz-muenchen.de
lab head
Sara Bilekova
contact affiliation	Helmholtz Zentrum München GmbH
contact email	sara.bilekova@helmholtz-munich.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043946
     1. Label: PRIDE project
     2. Name: Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility