PXD043946 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility |
Description | Spermatogenesis is a crucial biological process that enables the production of functional sperm, allowing for successful reproduction. Proper germ cell differentiation and maturation require tight regulation of hormonal signals, cellular signaling pathways, and cell biological processes. The acrosome is a lysosome-related organelle at the anterior of the sperm head that contains enzymes and receptors essential for egg-sperm recognition and fusion. Even though several factors crucial for acrosome biogenesis have been discovered, the precise molecular mechanism of pro-acrosomal vesicle formation and fusion is not known. In this study, we investigated the role of the insulin inhibitory receptor (inceptor) in acrosome formation. Inceptor is a single-pass transmembrane protein with similarities to mannose-6-phosphate receptors (M6PR). Inceptor knockout mice are infertile due to malformations in the acrosome and defects in the nuclear shape of spermatozoa. We show that inceptor is expressed in early spermatids and mainly localizes to vesicles between the Golgi apparatus and acrosome. We propose that inceptor is an important factor in the intracellular transport of trans-Golgi network (TGN)-derived clathrin-coated vesicles delivering acrosomal cargo in maturing spermatids, and its absence results in vesicle-fusion defects, acrosomal malformation, and male infertility. These findings support our hypothesis of inceptor as a universal lysosomal or lysosome-related organelle sorting receptor expressed in several secretory tissues. |
HostingRepository | PRIDE |
AnnounceDate | 2023-08-28 |
AnnouncementXML | Submission_2023-08-28_05:38:01.436.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | SaraBilekova |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-20 03:09:36 | ID requested | |
⏵ 1 | 2023-08-28 05:38:01 | announced | |
2 | 2023-11-14 07:26:32 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: vesicle, localization,acrosome, differentiation, spermatogenesis, male fertility |
Contact List
StefanieHauck |
contact affiliation | Metabolomics and Proteomics Core, Helmholtz Zentrum Muenchen |
contact email | hauck@helmholtz-muenchen.de |
lab head | |
SaraBilekova |
contact affiliation | Helmholtz Zentrum München GmbH |
contact email | sara.bilekova@helmholtz-munich.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD043946 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043946
- Label: PRIDE project
- Name: Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility