PXD043703-2

PXD043703 is an original dataset announced via ProteomeXchange.

Title	LC3A and Aggresomes: A trigger for UPR-activation, mitochondrial dysfunction, and cellular senescence
Description	The protein quality control (PQC) network is crucial for maintaining cellular proteostasis by monitoring protein production, folding, and degradation. Unfolded protein response (UPR) and autophagy are key components of this network along with the compartmentalization of misfolded proteins into inclusion bodies. Among inclusion bodies, aggresomes, a bimolecular condensate, formed by collapsed vimentin cage surrounding misfolded/aggregated proteins at the microtubule organizing center. LC3A, a member of the LC3 family of autophagy-related proteins, has been implicated in regulating protein homeostasis. In this study, we investigated the role of LC3A in aggresome-positive cells. Expression of LC3A-mediated autophagy was associated with activation of the PERK-eIF2-ATF4 axis of the UPR and alterations in mitochondrial morphology. Prolonged expression of LC3A induced cellular senescence in aggresome-positive cells. These findings provide insights into the specialized role of LC3A in cellular homeostasis. Understanding the molecular mechanisms underlying LC3A-mediated autophagy has implications for therapeutic strategies targeting protein quality control pathways.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:54:09.785.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nada Amer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-12 02:26:09	ID requested
1	2024-08-10 12:09:44	announced
⏵ 2	2024-10-22 06:54:17	announced	2024-10-22: Updated project metadata.

10.1016/j.jbc.2024.107398;

Amer N, Hesham D, Al-Shehaby N, Elshoky HA, Amer M, Magdeldin S, Mansour M, Abou-Aisha K, El-Naggar S, -ATF4 axis activation and mitochondrial dysfunction: Exposing vulnerability in aggresome-positive cancer cells. J Biol Chem, 300(6):107398(2024) [pubmed]

submitter keyword: Autophagy

Carcinoma

Choroid plexus

Endoplasmic reticulum

Inclusion bodies

MAP1LC3A

Mitochondria

Proteomics

Proteostasis

Senescence

Shahenda El-Naggar
contact affiliation	Tumor Biology Research Program, Research department, Children's Cancer Hospital in Egypt 57357
contact email	shahenda.elnaggar@57357.org
lab head
Nada Amer
contact affiliation	1- Tumor Biology Research Program, Basic Research Unit, Research Department, Children’s Cancer Hospital Egypt. 2- 2. Faculty of Pharmacy and Biotechnology, German University in Cairo (GUC), Egypt.
contact email	nada.amer@57357.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD043703

PRIDE project URI

• PRIDE
  1. PXD043703
     1. Label: PRIDE project
     2. Name: LC3A and Aggresomes: A trigger for UPR-activation, mitochondrial dysfunction, and cellular senescence