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PXD043703-1

PXD043703 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC3A and Aggresomes: A trigger for UPR-activation, mitochondrial dysfunction, and cellular senescence
DescriptionThe protein quality control (PQC) network is crucial for maintaining cellular proteostasis by monitoring protein production, folding, and degradation. Unfolded protein response (UPR) and autophagy are key components of this network along with the compartmentalization of misfolded proteins into inclusion bodies. Among inclusion bodies, aggresomes, a bimolecular condensate, formed by collapsed vimentin cage surrounding misfolded/aggregated proteins at the microtubule organizing center. LC3A, a member of the LC3 family of autophagy-related proteins, has been implicated in regulating protein homeostasis. In this study, we investigated the role of LC3A in aggresome-positive cells. Expression of LC3A-mediated autophagy was associated with activation of the PERK-eIF2-ATF4 axis of the UPR and alterations in mitochondrial morphology. Prolonged expression of LC3A induced cellular senescence in aggresome-positive cells. These findings provide insights into the specialized role of LC3A in cellular homeostasis. Understanding the molecular mechanisms underlying LC3A-mediated autophagy has implications for therapeutic strategies targeting protein quality control pathways.
HostingRepositoryPRIDE
AnnounceDate2024-08-10
AnnouncementXMLSubmission_2024-08-10_12:09:43.436.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNada Amer
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-12 02:26:09ID requested
12024-08-10 12:09:44announced
22024-10-22 06:54:17announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.jbc.2024.107398;
Amer N, Hesham D, Al-Shehaby N, Elshoky HA, Amer M, Magdeldin S, Mansour M, Abou-Aisha K, El-Naggar S, -ATF4 axis activation and mitochondrial dysfunction: Exposing vulnerability in aggresome-positive cancer cells. J Biol Chem, 300(6):107398(2024) [pubmed]
Keyword List
submitter keyword: Autophagy
Carcinoma
Choroid plexus
Endoplasmic reticulum
Inclusion bodies
MAP1LC3A
Mitochondria
Proteomics
Proteostasis
Senescence
Contact List
Shahenda El-Naggar
contact affiliationTumor Biology Research Program, Research department, Children's Cancer Hospital in Egypt 57357
contact emailshahenda.elnaggar@57357.org
lab head
Nada Amer
contact affiliation1- Tumor Biology Research Program, Basic Research Unit, Research Department, Children’s Cancer Hospital Egypt. 2- 2. Faculty of Pharmacy and Biotechnology, German University in Cairo (GUC), Egypt.
contact emailnada.amer@57357.org
dataset submitter
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