PXD043608-2

PXD043608 is an original dataset announced via ProteomeXchange.

Title	Therapeutic potential of pomegranate juice-derived nanovesicles in nude mouse benign prostatic hyperplasia (BPH) xenograft model
Description	Benign prostatic hyperplasia (BPH) and associated lower urinary tract symptoms affect a large percentage of the male population and places a substantial burden on the world health system. Current therapies include 5-alpha reductase inhibitors and alpha-blockers that are only partially effective and pose a huge economic burden, emphasizing the urgent need for effective, economical therapies. We isolated nanovesicles from pomegranate juice (Punica Granatum) (referred to as ‘POM-NVs’) and report to our knowledge for the first time, that these vesicles possess therapeutic potential against BPH. Following extensive characterization of POM-NVs, we tested their therapeutic potential in vitro using BPH1 cell line and identified a potential anti-proliferative and pro-apoptotic effect. We further tested these vesicles using a clinically relevant xenograft mouse BPH model derived from human BPH tissues. Remarkably, POM-NVs could reverse the BPH phenotype conferred by TGF-β mediated signaling and induced epithelial-to-mesenchymal (EMT) reversal, leading to the restoration of prostate epithelial states in vivo and in vitro. Furthermore, these vesicles attenuated bone morphogenic protein 5 (BMP5) signaling, a cardinal alteration that is instrumental in driving BPH. Considering the large incidences of BPH and its associated economic burdens, our study has important implications and can potentially improve the clinical management of BPH.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:54:15.104.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sharanjot Saini
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: 'Punica granatum' phytoplasma; NCBI TaxID: 1532734;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-07 09:58:19	ID requested
1	2023-07-18 10:16:01	announced
⏵ 2	2024-10-22 05:54:15	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Plant-derived nanovesicles, pomegranate juice derived nanovesicles, LC-MS/MS

Sharanjot Saini
contact affiliation	Augusta University
contact email	ssaini@augusta.edu
lab head
Sharanjot Saini
contact affiliation	Augusta University
contact email	ssaini@augusta.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043608
     1. Label: PRIDE project
     2. Name: Therapeutic potential of pomegranate juice-derived nanovesicles in nude mouse benign prostatic hyperplasia (BPH) xenograft model