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PXD043289-2

PXD043289 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDEAD box RNA helicases are pervasive protein kinase interactors and activators
DescriptionDEAD box (DDX) RNA helicases are a large family of ATPases, many of which have unknown functions. There is emerging evidence that besides their role in RNA biology, DDX proteins may stimulate protein kinases. To investigate if protein kinase-DDX interaction is a more widespread phenomenon, we conducted three orthogonal large-scale screens, including proteomics analysis with 32 RNA helicases, protein array profiling, and kinome-wide in vitro kinase assays. We retrieved Ser/Thr protein kinases as prominent interactors of RNA helicases and report hundreds of binary interactions. We extracted members of eleven protein kinase families, which bind to - and are stimulated by - DDX proteins, including CAMK, CDK, CK1, CK2, DYRK, MARK, NEK, PRKC, SPRK, STE7/MAP2K, and STE20/PAK family members. We identified MARK1 in all screens and validated that DDX proteins accelerate MARK1 catalytic rate. The findings indicate pervasive interactions between protein kinases and DEAD box RNA helicases and provide a rich resource to explore their regulatory relationships.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:32:43.838.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDimitris Papageorgiou
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-26 00:46:57ID requested
12024-03-10 10:10:03announced
22024-10-22 06:32:44announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Kinase activators,RNA helicases, Interactome
Contact List
Jeroen Krijgsveld
contact affiliationDivision head of Proteomics of Stem Cells and Cancer Deutsches Krebsforschungszentrum
contact emailj.krijgsveld@dkfz.de
lab head
Dimitris Papageorgiou
contact affiliationPost Doctoral Scientist
contact emaild.papageorgiou0@gmail.com
dataset submitter
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Dataset FTP location
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