PXD043289 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DEAD box RNA helicases are pervasive protein kinase interactors and activators |
Description | DEAD box (DDX) RNA helicases are a large family of ATPases, many of which have unknown functions. There is emerging evidence that besides their role in RNA biology, DDX proteins may stimulate protein kinases. To investigate if protein kinase-DDX interaction is a more widespread phenomenon, we conducted three orthogonal large-scale screens, including proteomics analysis with 32 RNA helicases, protein array profiling, and kinome-wide in vitro kinase assays. We retrieved Ser/Thr protein kinases as prominent interactors of RNA helicases and report hundreds of binary interactions. We extracted members of eleven protein kinase families, which bind to - and are stimulated by - DDX proteins, including CAMK, CDK, CK1, CK2, DYRK, MARK, NEK, PRKC, SPRK, STE7/MAP2K, and STE20/PAK family members. We identified MARK1 in all screens and validated that DDX proteins accelerate MARK1 catalytic rate. The findings indicate pervasive interactions between protein kinases and DEAD box RNA helicases and provide a rich resource to explore their regulatory relationships. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:32:43.838.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dimitris Papageorgiou |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-26 00:46:57 | ID requested | |
1 | 2024-03-10 10:10:03 | announced | |
⏵ 2 | 2024-10-22 06:32:44 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Kinase activators,RNA helicases, Interactome |
Contact List
Jeroen Krijgsveld |
contact affiliation | Division head of Proteomics of Stem Cells and Cancer Deutsches Krebsforschungszentrum |
contact email | j.krijgsveld@dkfz.de |
lab head | |
Dimitris Papageorgiou |
contact affiliation | Post Doctoral Scientist |
contact email | d.papageorgiou0@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043289
- Label: PRIDE project
- Name: DEAD box RNA helicases are pervasive protein kinase interactors and activators