PXD043289-1

PXD043289 is an original dataset announced via ProteomeXchange.

Title	DEAD box RNA helicases are pervasive protein kinase interactors and activators
Description	DEAD box (DDX) RNA helicases are a large family of ATPases, many of which have unknown functions. There is emerging evidence that besides their role in RNA biology, DDX proteins may stimulate protein kinases. To investigate if protein kinase-DDX interaction is a more widespread phenomenon, we conducted three orthogonal large-scale screens, including proteomics analysis with 32 RNA helicases, protein array profiling, and kinome-wide in vitro kinase assays. We retrieved Ser/Thr protein kinases as prominent interactors of RNA helicases and report hundreds of binary interactions. We extracted members of eleven protein kinase families, which bind to - and are stimulated by - DDX proteins, including CAMK, CDK, CK1, CK2, DYRK, MARK, NEK, PRKC, SPRK, STE7/MAP2K, and STE20/PAK family members. We identified MARK1 in all screens and validated that DDX proteins accelerate MARK1 catalytic rate. The findings indicate pervasive interactions between protein kinases and DEAD box RNA helicases and provide a rich resource to explore their regulatory relationships.
HostingRepository	PRIDE
AnnounceDate	2024-03-10
AnnouncementXML	Submission_2024-03-10_10:10:03.501.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dimitris Papageorgiou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-26 00:46:57	ID requested
⏵ 1	2024-03-10 10:10:03	announced
2	2024-10-22 06:32:44	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Kinase activators,RNA helicases, Interactome

Jeroen Krijgsveld
contact affiliation	Division head of Proteomics of Stem Cells and Cancer Deutsches Krebsforschungszentrum
contact email	j.krijgsveld@dkfz.de
lab head
Dimitris Papageorgiou
contact affiliation	Post Doctoral Scientist
contact email	d.papageorgiou0@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043289
     1. Label: PRIDE project
     2. Name: DEAD box RNA helicases are pervasive protein kinase interactors and activators