PXD042822-3

PXD042822 is an original dataset announced via ProteomeXchange.

Title	Cyclophilin A isomerization of Septin 2 mediates abscission during cytokinesis
Description	The isomerase activity of Cyclophilin A is important for midbody abscission during cell division, however to date, midbody substrates remain unknown. In this study, we report that the GTP-binding protein Septin 2 interacts with Cyclophilin A. We highlight a dynamic series of Septin 2 phenotypes at the midbody, previously undescribed in human cells. Furthermore, Cyclophilin A depletion or loss of isomerase activity is sufficient to induce phenotypic Septin 2 defects at the midbody. Structural and molecular analysis reveals that Septin 2 proline 259 is important for interaction with Cyclophilin A. Moreover, an isomerisation-deficient EGFP-Septin 2 proline 259 mutant displays defective midbody localisation, and undergoes impaired abscission, consistent with data from cells with loss of Cyclophilin A expression or activity. Collectively, this data reveals Septin 2 as a novel interacting partner and isomerase substrate of Cyclophilin A at the midbody that is required for abscission during cytokinesis in cancer cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:12:24.454.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Cotter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-08 04:45:43	ID requested
1	2023-10-24 13:21:43	announced
2	2023-11-14 09:11:44	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:12:25	announced	2024-10-22: Updated project metadata.

10.3390/ijms241311084;

Gorry RL, Brennan K, Lavin PTM, Mazurski T, Mary C, Matallanas D, Guichou JF, Mc Gee MM, Cyclophilin A Isomerisation of Septin 2 Mediates Abscission during Cytokinesis. Int J Mol Sci, 24(13):(2023) [pubmed]

submitter keyword: Human, Cancer,Cyclophilin A, Cytokinesis, Septin 2, K562 cells

Margaret M. McGee
contact affiliation	Associate Professor, University College Dublin, School of Biomolecular and Biomedical Science, Conway Institute Belfield Dublin 4
contact email	margaret.mcgee@ucd.ie
lab head
Philip Cotter
contact affiliation	University College Dublin
contact email	philip.cotter@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042822

PRIDE project URI

• PRIDE
  1. PXD042822
     1. Label: PRIDE project
     2. Name: Cyclophilin A isomerization of Septin 2 mediates abscission during cytokinesis