PXD042822-2

PXD042822 is an original dataset announced via ProteomeXchange.

Title	Cyclophilin A isomerization of Septin 2 mediates abscission during cytokinesis
Description	The isomerase activity of Cyclophilin A is important for midbody abscission during cell division, however to date, midbody substrates remain unknown. In this study, we report that the GTP-binding protein Septin 2 interacts with Cyclophilin A. We highlight a dynamic series of Septin 2 phenotypes at the midbody, previously undescribed in human cells. Furthermore, Cyclophilin A depletion or loss of isomerase activity is sufficient to induce phenotypic Septin 2 defects at the midbody. Structural and molecular analysis reveals that Septin 2 proline 259 is important for interaction with Cyclophilin A. Moreover, an isomerisation-deficient EGFP-Septin 2 proline 259 mutant displays defective midbody localisation, and undergoes impaired abscission, consistent with data from cells with loss of Cyclophilin A expression or activity. Collectively, this data reveals Septin 2 as a novel interacting partner and isomerase substrate of Cyclophilin A at the midbody that is required for abscission during cytokinesis in cancer cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:11:41.341.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Cotter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-08 04:45:43	ID requested
1	2023-10-24 13:21:43	announced
⏵ 2	2023-11-14 09:11:44	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:12:25	announced	2024-10-22: Updated project metadata.

Gorry RL, Brennan K, Lavin PTM, Mazurski T, Mary C, Matallanas D, Guichou JF, Mc Gee MM, Cyclophilin A Isomerisation of Septin 2 Mediates Abscission during Cytokinesis. Int J Mol Sci, 24(13):(2023) [pubmed]

submitter keyword: Human, Cancer,Cyclophilin A, Cytokinesis, Septin 2, K562 cells

Margaret M. McGee
contact affiliation	Associate Professor, University College Dublin, School of Biomolecular and Biomedical Science, Conway Institute Belfield Dublin 4
contact email	margaret.mcgee@ucd.ie
lab head
Philip Cotter
contact affiliation	University College Dublin
contact email	philip.cotter@ucd.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042822
     1. Label: PRIDE project
     2. Name: Cyclophilin A isomerization of Septin 2 mediates abscission during cytokinesis