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PXD042787-2

PXD042787 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of outer membrane vesicles released by clinical isolates of Neisseria gonorrhoeae
DescriptionThe sexually transmitted pathogen Neisseria gonorrhoeae releases outer membrane vesicles (OMVs) during infections. OMVs traffic the major porin PorB, other membrane proteins and lipo-oligosaccharide (LOS) into host innate immune cells and activate programmed cell death pathways and inflammation. Little is known, however, about the proteome and LOS content of OMVs released by clinical strains isolated from different infection sites, and whether this affects immune responses. Here, we characterized OMVs from four N. gonorrhoeae isolates and determined their size, abundance, proteome and activation of inflammatory responses in macrophages. The overall proteome of the OMVs was conserved between the four different isolates, included major outer membrane, periplasm, cytoplasmic membrane proteins. Despite this, we observed differences in the rate of OMV biogenesis and the relative abundance of major outer membrane proteins and LOS. Consequently, OMVs from clinical isolates induced varying rates of macrophage cell death and the secretion of interleukin-1 family members, such as Il-1andIl-1. Overall, these findings demonstrate that clinical isolates of N. gonorrhoeae utilize OMVs to release major proteins and lipids, which affects innate immune responses.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:56:37.045.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRalf Schittenhelm
SpeciesList scientific name: Neisseria gonorrhoeae; NCBI TaxID: 485;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-07 02:55:54ID requested
12024-08-26 16:53:41announced
22024-10-22 06:56:37announced2024-10-22: Updated project metadata.
Publication List
10.1002/PMIC.202300087;
Keyword List
submitter keyword: macrophage, proteomics, inflammation, inflammasome,OMV, gonorrhoea
Contact List
Thomas Naderer
contact affiliationDepartment of Biochemistry & Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton 3800, Australia
contact emailThomas.naderer@monash.edu
lab head
Ralf Schittenhelm
contact affiliationMonash University
contact emailralf.schittenhelm@monash.edu
dataset submitter
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