PXD042787-1

PXD042787 is an original dataset announced via ProteomeXchange.

Title	Characterization of outer membrane vesicles released by clinical isolates of Neisseria gonorrhoeae
Description	The sexually transmitted pathogen Neisseria gonorrhoeae releases outer membrane vesicles (OMVs) during infections. OMVs traffic the major porin PorB, other membrane proteins and lipo-oligosaccharide (LOS) into host innate immune cells and activate programmed cell death pathways and inflammation. Little is known, however, about the proteome and LOS content of OMVs released by clinical strains isolated from different infection sites, and whether this affects immune responses. Here, we characterized OMVs from four N. gonorrhoeae isolates and determined their size, abundance, proteome and activation of inflammatory responses in macrophages. The overall proteome of the OMVs was conserved between the four different isolates, included major outer membrane, periplasm, cytoplasmic membrane proteins. Despite this, we observed differences in the rate of OMV biogenesis and the relative abundance of major outer membrane proteins and LOS. Consequently, OMVs from clinical isolates induced varying rates of macrophage cell death and the secretion of interleukin-1 family members, such as Il-1andIl-1. Overall, these findings demonstrate that clinical isolates of N. gonorrhoeae utilize OMVs to release major proteins and lipids, which affects innate immune responses.
HostingRepository	PRIDE
AnnounceDate	2024-08-27
AnnouncementXML	Submission_2024-08-26_16:53:41.263.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ralf Schittenhelm
SpeciesList	scientific name: Neisseria gonorrhoeae; NCBI TaxID: 485;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-07 02:55:54	ID requested
⏵ 1	2024-08-26 16:53:41	announced
2	2024-10-22 06:56:37	announced	2024-10-22: Updated project metadata.

10.1002/PMIC.202300087;

submitter keyword: macrophage, proteomics, inflammation, inflammasome,OMV, gonorrhoea

Thomas Naderer
contact affiliation	Department of Biochemistry & Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton 3800, Australia
contact email	Thomas.naderer@monash.edu
lab head
Ralf Schittenhelm
contact affiliation	Monash University
contact email	ralf.schittenhelm@monash.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042787
     1. Label: PRIDE project
     2. Name: Characterization of outer membrane vesicles released by clinical isolates of Neisseria gonorrhoeae