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PXD042704 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEvaluating the Performance of the Astral Mass Analyzer for Quantitative Proteomics Using Data Independent Acquisition
DescriptionWe evaluate the quantitative performance of the newly released Asymmetric Track Lossless (Astral) analyzer. Using data independent acquisition, the Orbitrap Astral mass spectrometer quantifies 5 times more peptides per unit time than state-of-the-art Orbitrap mass spectrometers, which have long been the gold standard for high resolution quantitative proteomics. Our results demonstrate that the Orbitrap Astral mass spectrometer is capable of producing high quality quantitative measurements across a wide dynamic range. We also use a newly developed extra-cellular vesicle enrichment protocol to reach new depths of coverage in the plasma proteome, quantifying over 5,000 plasma proteins in a 60-minute gradient with the Orbitrap Astral mass spectrometer.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLilian Heil
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
InstrumentOrbitrap Fusion Lumos; Orbitrap Astral
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-02 22:24:29ID requested
12023-06-18 01:01:51announced
22023-09-18 21:36:12announced: Added PubMed Id
Publication List
Heil LR, Damoc NE, Arrey TN, Pashkova A, Denisov E, Petzoldt J, Peterson A, Hsu C, Searle BC, Shulman N, Riffle M, Connolly B, MacLean BX, Remes PM, Senko M, Stewart H, Hock C, Makarov A, Hermanson D, Zabrouskov V, Wu CC, MacCoss MJ. Evaluating the performance of the Astral mass analyzer for quantitative proteomics using data independent acquisition. bioRxiv [Internet]. 2023 Jan 1;2023.06.03.543570. Available from: http://biorxiv.org/content/early/2023/06/03/2023.06.03.543570.abstract
Keyword List
submitter keyword: plasma, DIA, Astral, Orbitrap, limit of quantification
Contact List
Michael MacCoss
contact affiliationUniversity of Washington
contact emailmaccoss@uw.edu
lab head
Lilian Heil
contact affiliationUniversity of Washington
contact emaillheil2@uw.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI