PXD042704-2
PXD042704 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Evaluating the Performance of the Astral Mass Analyzer for Quantitative Proteomics Using Data Independent Acquisition |
| Description | We evaluate the quantitative performance of the newly released Asymmetric Track Lossless (Astral) analyzer. Using data independent acquisition, the Orbitrap Astral mass spectrometer quantifies 5 times more peptides per unit time than state-of-the-art Orbitrap mass spectrometers, which have long been the gold standard for high resolution quantitative proteomics. Our results demonstrate that the Orbitrap Astral mass spectrometer is capable of producing high quality quantitative measurements across a wide dynamic range. We also use a newly developed extra-cellular vesicle enrichment protocol to reach new depths of coverage in the plasma proteome, quantifying over 5,000 plasma proteins in a 60-minute gradient with the Orbitrap Astral mass spectrometer. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2023-09-18 |
| AnnouncementXML | Submission_2023-09-18_21:36:11.424.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Lilian Heil |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl |
| Instrument | Orbitrap Fusion Lumos; Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-06-02 22:24:29 | ID requested | |
| 1 | 2023-06-18 01:01:51 | announced | |
| ⏵ 2 | 2023-09-18 21:36:12 | announced | : Added PubMed Id |
Publication List
| Heil LR, Damoc E, Arrey TN, Pashkova A, Denisov E, Petzoldt J, Peterson AC, Hsu C, Searle BC, Shulman N, Riffle M, Connolly B, MacLean BX, Remes PM, Senko MW, Stewart HI, Hock C, Makarov AA, Hermanson D, Zabrouskov V, Wu CC, MacCoss MJ, Evaluating the Performance of the Astral Mass Analyzer for Quantitative Proteomics Using Data-Independent Acquisition. J Proteome Res, 22(10):3290-3300(2023) [pubmed] |
Keyword List
| submitter keyword: plasma, DIA, Astral, Orbitrap, limit of quantification |
Contact List
| Michael MacCoss | |
|---|---|
| contact affiliation | University of Washington |
| contact email | maccoss@uw.edu |
| lab head | |
| Lilian Heil | |
| contact affiliation | University of Washington |
| contact email | lheil2@uw.edu |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
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