PXD042371 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | miR-124 coordinates metabolic regulators acting at early stages of human neurogenesis |
| Description | Metabolic dysregulation of neurons is associated with diverse human brain disorders. Metabolic reprogramming occurs during neuronal differentiation, but it is not fully understood which molecules regulate metabolic changes at the early stages of neurogenesis. In this study, we report that miR-124 is a driver of metabolic change at the initiating stage of human neurogenesis. Proteome analysis has shown the oxidative phosphorylation pathway to be the most significantly altered among the differentially expressed proteins (DEPs) in the immature neurons after the knockdown of miR-124. In agreement with these proteomics results, miR-124-depleted neurons display mitochondrial dysfunctions, such as decreased mitochondrial membrane potential and cellular respiration. Moreover, morphological analyses of mitochondria in early differentiated neurons after miR-124 knockdown result in smaller and less mature shapes. Lastly, we show the potential of identified DEPs as novel metabolic regulators in early neuronal development by validating the effects of GSTK1 on cellular respiration. GSTK1, which is upregulated most significantly in miR-124 knockdown neurons, reduces the oxygen consumption rate of neural cells. Collectively, our data highlight the roles of miR-124 in coordinating metabolic maturation at the early stages of neurogenesis and provide insights into potential metabolic regulators associated with human brain disorders characterised by metabolic dysfunctions. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-28 |
| AnnouncementXML | Submission_2024-10-28_02:31:38.131.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yongwoo Na |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-05-21 00:43:27 | ID requested | |
| 1 | 2024-10-15 01:50:10 | announced | |
| 2 | 2024-10-16 03:50:19 | announced | 2024-10-16: Updated project metadata. |
| 3 | 2024-10-22 07:01:27 | announced | 2024-10-22: Updated project metadata. |
| ⏵ 4 | 2024-10-28 02:31:39 | announced | 2024-10-28: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: miRNA, nerogenesis,human |
Contact List
| Jinju Han |
|---|
| contact affiliation | Graduate School of Medical Science and Engineering, Korea Advanced Institute for Science and Technology (KAIST), Daejeon, 34141, Korea |
| contact email | jinjuhan@kaist.ac.kr |
| lab head | |
| Yongwoo Na |
|---|
| contact affiliation | Seoul National University |
| contact email | nyw1412@snu.ac.kr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042371
- Label: PRIDE project
- Name: miR-124 coordinates metabolic regulators acting at early stages of human neurogenesis
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