PXD042371-1

PXD042371 is an original dataset announced via ProteomeXchange.

Title	miR-124 coordinates metabolic regulators working at the early human neurogenesis
Description	Metabolic dysregulation of neurons is associated with diverse human brain disorders. Metabolic reprogramming occurs during neuronal differentiation, but it is not fully understood which molecules regulate metabolic changes at the early stages of neurogenesis. In this study, we report miR-124 as a driver of metabolic change at the initiating stage of human neurogenesis. Proteome analysis has shown the oxidative phosphorylation pathway as the most significantly altered among the differentially expressed proteins (DEPs) in the immature neurons after the knockdown of miR-124. In agreement with the proteomics results, miR-124 depleted neurons displayed mitochondrial dysfunctions, such as decreased mitochondrial membrane potential and cellular respiration. Moreover, morphological analyses of mitochondria in early differentiated neurons after miR-124 knockdown resulted in fragmented shapes. Lastly, we showed the potential of DEPs as metabolic regulators in neuronal development by validating the effects of GSTK1 on cellular respiration. GSTK1, upregulated most significantly in miR-124 knockdown neurons, reduces oxygen consumption rate of neural cells. Collectively, our data demonstrate and emphasize the roles of miR-124 in coordinating metabolic maturation at the early stage of neurogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-15
AnnouncementXML	Submission_2024-10-15_01:50:09.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yongwoo Na
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-21 00:43:27	ID requested
⏵ 1	2024-10-15 01:50:10	announced
2	2024-10-16 03:50:19	announced	2024-10-16: Updated project metadata.
3	2024-10-22 07:01:27	announced	2024-10-22: Updated project metadata.
4	2024-10-28 02:31:39	announced	2024-10-28: Updated project metadata.

Dataset with its publication pending

submitter keyword: miRNA, nerogenesis,human

Jinju Han
contact affiliation	Graduate School of Medical Science and Engineering, Korea Advanced Institute for Science and Technology (KAIST), Daejeon, 34141, Korea
contact email	jinjuhan@kaist.ac.kr
lab head
Yongwoo Na
contact affiliation	Seoul National University
contact email	nyw1412@snu.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD042371

PRIDE project URI

• PRIDE
  1. PXD042371
     1. Label: PRIDE project
     2. Name: miR-124 coordinates metabolic regulators working at the early human neurogenesis