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PXD042371-1

PXD042371 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlemiR-124 coordinates metabolic regulators working at the early human neurogenesis
DescriptionMetabolic dysregulation of neurons is associated with diverse human brain disorders. Metabolic reprogramming occurs during neuronal differentiation, but it is not fully understood which molecules regulate metabolic changes at the early stages of neurogenesis. In this study, we report miR-124 as a driver of metabolic change at the initiating stage of human neurogenesis. Proteome analysis has shown the oxidative phosphorylation pathway as the most significantly altered among the differentially expressed proteins (DEPs) in the immature neurons after the knockdown of miR-124. In agreement with the proteomics results, miR-124 depleted neurons displayed mitochondrial dysfunctions, such as decreased mitochondrial membrane potential and cellular respiration. Moreover, morphological analyses of mitochondria in early differentiated neurons after miR-124 knockdown resulted in fragmented shapes. Lastly, we showed the potential of DEPs as metabolic regulators in neuronal development by validating the effects of GSTK1 on cellular respiration. GSTK1, upregulated most significantly in miR-124 knockdown neurons, reduces oxygen consumption rate of neural cells. Collectively, our data demonstrate and emphasize the roles of miR-124 in coordinating metabolic maturation at the early stage of neurogenesis.
HostingRepositoryPRIDE
AnnounceDate2024-10-15
AnnouncementXMLSubmission_2024-10-15_01:50:09.528.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYongwoo Na
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-21 00:43:27ID requested
12024-10-15 01:50:10announced
22024-10-16 03:50:19announced2024-10-16: Updated project metadata.
32024-10-22 07:01:27announced2024-10-22: Updated project metadata.
42024-10-28 02:31:39announced2024-10-28: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: miRNA, nerogenesis,human
Contact List
Jinju Han
contact affiliationGraduate School of Medical Science and Engineering, Korea Advanced Institute for Science and Technology (KAIST), Daejeon, 34141, Korea
contact emailjinjuhan@kaist.ac.kr
lab head
Yongwoo Na
contact affiliationSeoul National University
contact emailnyw1412@snu.ac.kr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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