PXD042366-2

PXD042366 is an original dataset announced via ProteomeXchange.

Title	Secretome analysis of breast cancer cells treated with Ipomoea turpethum extract-loaded polymeric nanoparticles.
Description	Nanoparticle-based formulations bypass biological barriers, allowing for longer blood circulation times, simultaneous tumor targeting, and increased drug accumulation in tumors. They have a higher therapeutic efficiency than traditional treatment due to better specificity for effective drug targeting, no solubility, and stability issues. In our previous study, we showed that Ipomoea turpethum extract-loaded polymeric nanoparticles induce cytotoxicity in breast cancer cell lines (MCF-7 and MDA MB-231) by regulating pathways involved in apoptosis and cell proliferation 9. Many researchers demonstrated the use of tumor microenvironments for prognostic, diagnostics, and therapeutic purposes. In the present study, we have analyzed the effect of NVA-IT treatment on the tumor microenvironment via secretory proteins present in the medium of the tumor cells. We have used Nano LCMS/MS to identify the differentially expressed proteins present in the culture medium of the NVA-IT treated tumor cells compared to untreated tumor cells. The differentially expressed proteins were further analyzed using bioinformatics tools.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:26:25.917.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SAIMA WAJID
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-20 03:33:26	ID requested
1	2023-10-10 00:31:13	announced
⏵ 2	2023-11-14 08:26:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Bioinformatics., Nano LCMS/MS, Ipomoea turpethum, Nanoparticles, Secretome analysis,Breast Cancer

Dr. Saima Wajid
contact affiliation	Department of Biotechnology, School of Chemical & Life Sciences, Jamia Hamdard, Hamdard Nagar, New Delhi, 110062, India
contact email	swajid@jamiahamdard.ac.in
lab head
SAIMA WAJID
contact affiliation	Department of Biotechnology, School of Chemical & Life Sciences, Jamia Hamdard, Hamdard Nagar, New Delhi, 110062.
contact email	swajid@jamiahamdard.ac.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042366
     1. Label: PRIDE project
     2. Name: Secretome analysis of breast cancer cells treated with Ipomoea turpethum extract-loaded polymeric nanoparticles.