PXD041975-2

PXD041975 is an original dataset announced via ProteomeXchange.

Title	Identification of the protein interaction network of STAG2 protein isoforms with inclusion and exclusion of exon32
Description	This study involves the identification of specific interactors for STAG2ex32+ and STAG2ex32delta. Plasmids expressing YFP-STAG2 isoforms were transfected into HCT116OsTIR cells using untransfected cells as mock control. 40h post transfection, whole cell lysates were extracted and STAG2 isoforms were immunoprecipiated using GFP-TRAP. An unspecific GFP-TRAP pulldown was also conducted using lysates from untransfected cells to assess for unspecific protein binding to the beads (Mock). Significant proteins were determined by label-free quantification and statistical analysis using MaxQuant and MSstats.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:28.696.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Silvia Surinova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-04 02:37:40	ID requested
1	2023-05-04 03:27:46	announced
⏵ 2	2023-11-14 08:45:38	announced	2023-11-14: Updated project metadata.

Porter H, Li Y, Neguembor MV, Beltran M, Varsally W, Martin L, Cornejo MT, Pezi, ć D, Bhamra A, Surinova S, Jenner RG, Cosma MP, Hadjur S, Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading. Elife, 12():(2023) [pubmed]

submitter keyword: AP-MS

Silvia Surinova
contact affiliation	UCL Cancer Institute, London, UK
contact email	s.surinova@ucl.ac.uk
lab head
Silvia Surinova
contact affiliation	UCL
contact email	s.surinova@ucl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD041975

PRIDE project URI

• PRIDE
  1. PXD041975
     1. Label: PRIDE project
     2. Name: Identification of the protein interaction network of STAG2 protein isoforms with inclusion and exclusion of exon32