PXD041975-1

PXD041975 is an original dataset announced via ProteomeXchange.

Title	Identification of the protein interaction network of STAG2 protein isoforms with inclusion and exclusion of exon32
Description	This study involves the identification of specific interactors for STAG2ex32+ and STAG2ex32delta. Plasmids expressing YFP-STAG2 isoforms were transfected into HCT116OsTIR cells using untransfected cells as mock control. 40h post transfection, whole cell lysates were extracted and STAG2 isoforms were immunoprecipiated using GFP-TRAP. An unspecific GFP-TRAP pulldown was also conducted using lysates from untransfected cells to assess for unspecific protein binding to the beads (Mock). Significant proteins were determined by label-free quantification and statistical analysis using MaxQuant and MSstats.
HostingRepository	PRIDE
AnnounceDate	2023-05-04
AnnouncementXML	Submission_2023-05-04_03:27:45.311.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SilviaSurinova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-04 02:37:40	ID requested
⏵ 1	2023-05-04 03:27:46	announced
2	2023-11-14 08:45:38	announced	2023-11-14: Updated project metadata.

submitter keyword: AP-MS

SilviaSurinova
contact affiliation	UCL Cancer Institute, London, UK
contact email	s.surinova@ucl.ac.uk
lab head
SilviaSurinova
contact affiliation	UCL
contact email	s.surinova@ucl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041975
     1. Label: PRIDE project
     2. Name: Identification of the protein interaction network of STAG2 protein isoforms with inclusion and exclusion of exon32