PXD040306 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Depletion and reconstitution of 5S RNP complexes in early pre60S ribosomes |
Description | We purified pre60S ribosomes from yeast using Nsa1-FtpA as bait in Tandem Affinity Purification experiments. We tested the depletion of RPF2, using a Galactose-depletion system, to obtain ribosomal particles depleted of 5S RNP complexes. Sample A1: Control group (wild type strain) grown in YPGal; Sample A2: Control group (wild type strain) grown in YPGlu; Sample A3: Control group (Gal::HA-RPF2 strain) grown in YPGal; and Sample A4: Test group (Gal::HA-RPF2) shifted from YPGal to YPGlu for 6 hours to achieve 5S RNP depletion. Sample N1: Purified pre-60S Nsa1-associated with 5S RNP depleted after Sucrose gradient separation; Sample N2: Purified pre-60S Nsa1-associated with 5S RNP reconstituted after Sucrose gradient separation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:08:27.230.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Douglas Lamont |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-21 02:44:19 | ID requested | |
1 | 2023-10-24 11:04:46 | announced | |
2 | 2023-11-14 09:07:17 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 06:08:28 | announced | 2024-10-22: Updated project metadata. |
Publication List
Castillo Duque de Estrada NM, Thoms M, Flemming D, Hammaren HM, Buschauer R, Ameismeier M, Ba, ß, ler J, Beck M, Beckmann R, Hurt E, Structure of nascent 5S RNPs at the crossroad between ribosome assembly and MDM2-p53 pathways. Nat Struct Mol Biol, 30(8):1119-1131(2023) [pubmed] |
10.1038/s41594-023-01006-7; |
Keyword List
submitter keyword: 5S RNP, ribosomes, reconstitution, depletion, pre60S |
Contact List
Professor Ed Hurt |
contact affiliation | Heidelberg University Biochemistry Center (BZH) Im Neuenheimer Feld 328 69120 Heidelberg |
contact email | ed.hurt@bzh.uni-heidelberg.de |
lab head | |
Douglas Lamont |
contact affiliation | School of Life Sciences |
contact email | d.j.lamont@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040306
- Label: PRIDE project
- Name: Depletion and reconstitution of 5S RNP complexes in early pre60S ribosomes