PXD040306-1

PXD040306 is an original dataset announced via ProteomeXchange.

Title	Depletion and reconstitution of 5S RNP complexes in early pre60S ribosomes
Description	We purified pre60S ribosomes from yeast using Nsa1-FtpA as bait in Tandem Affinity Purification experiments. We tested the depletion of RPF2, using a Galactose-depletion system, to obtain ribosomal particles depleted of 5S RNP complexes. Sample A1: Control group (wild type strain) grown in YPGal; Sample A2: Control group (wild type strain) grown in YPGlu; Sample A3: Control group (Gal::HA-RPF2 strain) grown in YPGal; and Sample A4: Test group (Gal::HA-RPF2) shifted from YPGal to YPGlu for 6 hours to achieve 5S RNP depletion. Sample N1: Purified pre-60S Nsa1-associated with 5S RNP depleted after Sucrose gradient separation; Sample N2: Purified pre-60S Nsa1-associated with 5S RNP reconstituted after Sucrose gradient separation.
HostingRepository	PRIDE
AnnounceDate	2023-10-24
AnnouncementXML	Submission_2023-10-24_11:04:45.734.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DouglasLamont
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-21 02:44:19	ID requested
⏵ 1	2023-10-24 11:04:46	announced
2	2023-11-14 09:07:17	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:08:28	announced	2024-10-22: Updated project metadata.

Castillo Duque de Estrada NM, Thoms M, Flemming D, Hammaren HM, Buschauer R, Ameismeier M, Ba, ß, ler J, Beck M, Beckmann R, Hurt E, Structure of nascent 5S RNPs at the crossroad between ribosome assembly and MDM2-p53 pathways. Nat Struct Mol Biol, 30(8):1119-1131(2023) [pubmed]

submitter keyword: 5S RNP, ribosomes, reconstitution, depletion, pre60S

Professor EdHurt
contact affiliation	Heidelberg University Biochemistry Center (BZH) Im Neuenheimer Feld 328 69120 Heidelberg
contact email	ed.hurt@bzh.uni-heidelberg.de
lab head
DouglasLamont
contact affiliation	School of Life Sciences
contact email	d.j.lamont@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD040306

PRIDE project URI

• PRIDE
  1. PXD040306
     1. Label: PRIDE project
     2. Name: Depletion and reconstitution of 5S RNP complexes in early pre60S ribosomes