PXD040304-2

PXD040304 is an original dataset announced via ProteomeXchange.

Title	The Daphnia cuticle proteome - partial cuticle samples
Description	The cuticles of arthropods, including aquatic crustaceans like Daphnia, provide an interface between the organism and its environment. Thus, the cuticle’s structure influences how the organism responds to and interacts with its surroundings. Here, we used label-free quantification proteomics to provide a proteome of the molted cuticle of Daphnia magna, which has long been a prominent subject of studies on ecology, evolution, and developmental biology, anddetected 278 high confidence proteins. Using protein sequence domain and functional enrichment analyses, we identified chitin-binding structural proteins and chitin modifying enzymes as most abundant protein groups in the cuticle proteome.Structural cuticular protein families showed a similar distribution to those found in other arthropods and indicated proteins responsible for the soft and flexible structure of the Daphnia cuticle . Finally, cuticle protein genes were clustered as tandem gene arrays in the Daphnia genome, indicating their importance for adaptation to environmental change. The cuticle proteome presented here will be a valuable resource to the Daphnia research community, informing investigations on diverse topics such as the genetic basis of interactions with predators and parasites.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:57:37.846.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathrin Otte
SpeciesList	scientific name: Daphnia magna; NCBI TaxID: 35525;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-21 02:43:37	ID requested
1	2024-09-02 05:26:38	announced
⏵ 2	2024-10-22 06:57:38	announced	2024-10-22: Updated project metadata.

10.1002/PMIC.202300292;

submitter keyword: Arthropods

Cuticle

Daphnia

mass spectrometry

label-free quantification proteomics

Kathrin Otte
contact affiliation	University of Cologne, Germany
contact email	kathrin.anna.otte@gmail.com
lab head
Kathrin Otte
contact affiliation	Institute of Cell and Systems Biology of Animals, University of Hamburg, Hamburg, Germany
contact email	kathrin.anna.otte@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD040304

PRIDE project URI

• PRIDE
  1. PXD040304
     1. Label: PRIDE project
     2. Name: The Daphnia cuticle proteome - partial cuticle samples