PXD040115-2

PXD040115 is an original dataset announced via ProteomeXchange.

Title	Proteomics coupled with AhR-reporter gene bioassay for human and environmental safety assessment of sewage sludge and hydrochar
Description	BACKGROUND: Sewage sludge (SL) and hydrochar (HC) agricultural applications are nowadays a common practice to soil conditioning and crop fertilization, however safety concerns for human and environmental health due to the presence of toxic compounds, have been recently increased. OBJECTIVES: Our aim is to assess the safety for humans and the environment of SL and derived HC applications. METHODS: We applied proteomics and bioinformatics analyses to the cell cultures used for DR-CALUX® bioassay to identify differentially abundant proteins regulated upon exposure to SL and derived HC samples rather than only using the Bioanalytical Toxicity Equivalents (BEQs) obtained by the DR- CALUX® system itself. RESULTS: DR-CALUX® cells exposed to SL or HC showed a differential protein pattern of abundance dependent on the SL and HC extracts. The modified proteins were involved in antioxidant pathways, unfolded protein response and DNA damage that have close correlations with dioxin effects on the biological system and with cancer and neurological disorder onset. Moreover, other evidence suggests the enrichment of heavy metals in extracts according to cellular response. DISCUSSION: This work represents the next step of CALUX bioassay for screening polyhalogenated dibenzo-p-dioxins/furans and dioxin-like polychlorinated biphenyls in HC and SL and confirm to be successful in screening proteins which abundance is dependent on the SL and HC extracts in addition to the occurrence and biological activity of legacy toxic compounds not only belonging to organohalogens but still detectable for their biological activity in both matrices.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:52:27.076.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Enxhi Shaba
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-14 02:52:04	ID requested
1	2023-06-26 08:30:12	announced
⏵ 2	2023-11-14 06:52:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: sludge

hydrochar

CALUX

unfolded protein response

DNA damage

dioxin

Prof. Luca Bini
contact affiliation	Functional Proteomics Lab, Department of Life Science, University of Siena, Siena, Italy
contact email	luca.bini@unisi.it
lab head
Enxhi Shaba
contact affiliation	University of Siena
contact email	shaba3@student.unisi.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD040115

PRIDE project URI

• PRIDE
  1. PXD040115
     1. Label: PRIDE project
     2. Name: Proteomics coupled with AhR-reporter gene bioassay for human and environmental safety assessment of sewage sludge and hydrochar