PXD040115 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics coupled with AhR-reporter gene bioassay for human and environmental safety assessment of sewage sludge and hydrochar |
Description | BACKGROUND: Sewage sludge (SL) and hydrochar (HC) agricultural applications are nowadays a common practice to soil conditioning and crop fertilization, however safety concerns for human and environmental health due to the presence of toxic compounds, have been recently increased. OBJECTIVES: Our aim is to assess the safety for humans and the environment of SL and derived HC applications. METHODS: We applied proteomics and bioinformatics analyses to the cell cultures used for DR-CALUX® bioassay to identify differentially abundant proteins regulated upon exposure to SL and derived HC samples rather than only using the Bioanalytical Toxicity Equivalents (BEQs) obtained by the DR- CALUX® system itself. RESULTS: DR-CALUX® cells exposed to SL or HC showed a differential protein pattern of abundance dependent on the SL and HC extracts. The modified proteins were involved in antioxidant pathways, unfolded protein response and DNA damage that have close correlations with dioxin effects on the biological system and with cancer and neurological disorder onset. Moreover, other evidence suggests the enrichment of heavy metals in extracts according to cellular response. DISCUSSION: This work represents the next step of CALUX bioassay for screening polyhalogenated dibenzo-p-dioxins/furans and dioxin-like polychlorinated biphenyls in HC and SL and confirm to be successful in screening proteins which abundance is dependent on the SL and HC extracts in addition to the occurrence and biological activity of legacy toxic compounds not only belonging to organohalogens but still detectable for their biological activity in both matrices. |
HostingRepository | PRIDE |
AnnounceDate | 2023-06-26 |
AnnouncementXML | Submission_2023-06-26_08:30:11.978.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | EnxhiShaba |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | No PTMs are included in the dataset |
Instrument | autoflex |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-14 02:52:04 | ID requested | |
⏵ 1 | 2023-06-26 08:30:12 | announced | |
2 | 2023-11-14 06:52:27 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: sludge |
hydrochar |
CALUX |
unfolded protein response |
DNA damage |
dioxin |
Contact List
Prof. LucaBini |
contact affiliation | Functional Proteomics Lab, Department of Life Science, University of Siena, Siena, Italy |
contact email | luca.bini@unisi.it |
lab head | |
EnxhiShaba |
contact affiliation | University of Siena |
contact email | shaba3@student.unisi.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD040115 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040115
- Label: PRIDE project
- Name: Proteomics coupled with AhR-reporter gene bioassay for human and environmental safety assessment of sewage sludge and hydrochar