PXD039477-2

PXD039477 is an original dataset announced via ProteomeXchange.

Title	ASCL2 is a key regulator of the proliferation-differentiation equilibrium in the esophageal epithelium
Description	The esophagus is protected from the hostile environment by a stratified epithelium, which renews rapidly. Homeostasis of this epithelium is ensured by a rare population of stem cells in the basal layer: Keratin 15+ (Krt15+) cells. However, little is known about the molecular mechanisms regulating their distinct features, namely self-renewal, potency, and epithelial regeneration. Achaete-Scute Family BHLH Transcription Factor 2 (ASCL2) is strongly upregulated in Krt15+ stem cells and is known to contribute to stem cell maintenance in other tissues. Herein, we investigated the role of ASCL2 in maintaining homeostasis under normal and stress conditions in the esophageal epithelium. ASCL2 overexpression severely dysregulated cell differentiation and cell fate. Proliferation was also reduced due potentially to a blockage in the G1 phase of the cell cycle or an induction of quiescence. Mass spectrometry analysis confirmed alterations in several proteins associated with differentiation and cell cycle. In addition, overexpression of ASCL2 enhanced resistance to radiation and chemotherapeutic drugs. Overall, these results denoted the role of ASCL2 as a key regulator of the proliferation-differentiation equilibrium in the esophageal epithelium.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:35.309.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maude Hamilton
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-16 08:29:03	ID requested
1	2024-05-24 02:20:10	announced
⏵ 2	2024-10-22 06:42:36	announced	2024-10-22: Updated project metadata.

Hamilton M, Mars Z, Sedeuil M, Rolland M, Jean D, Boudreau F, Giroux V, ASCL2 is a key regulator of the proliferation-differentiation equilibrium in the esophageal epithelium. Biol Open, 13(1):(2024) [pubmed]

10.1242/bio.059919;

submitter keyword: esophagus, differentiation, stem cells,ASCL2, proliferation

Véronique Giroux
contact affiliation	Université de Sherbrooke
contact email	veronique.giroux@usherbrooke.ca
lab head
Maude Hamilton
contact affiliation	Department of Immunology and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Canada
contact email	maude.hamilton@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD039477

PRIDE project URI

• PRIDE
  1. PXD039477
     1. Label: PRIDE project
     2. Name: ASCL2 is a key regulator of the proliferation-differentiation equilibrium in the esophageal epithelium