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PXD039477-1

PXD039477 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleASCL2 is a key regulator of the proliferation-differentiation equilibrium in the esophageal epithelium
DescriptionThe esophagus is protected from the hostile environment by a stratified epithelium, which renews rapidly. Homeostasis of this epithelium is ensured by a rare population of stem cells in the basal layer: Keratin 15+ (Krt15+) cells. However, little is known about the molecular mechanisms regulating their distinct features, namely self-renewal, potency, and epithelial regeneration. Achaete-Scute Family BHLH Transcription Factor 2 (ASCL2) is strongly upregulated in Krt15+ stem cells and is known to contribute to stem cell maintenance in other tissues. Herein, we investigated the role of ASCL2 in maintaining homeostasis under normal and stress conditions in the esophageal epithelium. ASCL2 overexpression severely dysregulated cell differentiation and cell fate. Proliferation was also reduced due potentially to a blockage in the G1 phase of the cell cycle or an induction of quiescence. Mass spectrometry analysis confirmed alterations in several proteins associated with differentiation and cell cycle. In addition, overexpression of ASCL2 enhanced resistance to radiation and chemotherapeutic drugs. Overall, these results denoted the role of ASCL2 as a key regulator of the proliferation-differentiation equilibrium in the esophageal epithelium.
HostingRepositoryPRIDE
AnnounceDate2024-05-24
AnnouncementXMLSubmission_2024-05-24_02:20:09.506.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaude Hamilton
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-16 08:29:03ID requested
12024-05-24 02:20:10announced
22024-10-22 06:42:36announced2024-10-22: Updated project metadata.
Publication List
Hamilton M, Mars Z, Sedeuil M, Rolland M, Jean D, Boudreau F, Giroux V, ASCL2 is a key regulator of the proliferation-differentiation equilibrium in the esophageal epithelium. Biol Open, 13(1):(2024) [pubmed]
10.1242/bio.059919;
Keyword List
submitter keyword: esophagus, differentiation, stem cells,ASCL2, proliferation
Contact List
Véronique Giroux
contact affiliationUniversité de Sherbrooke
contact emailveronique.giroux@usherbrooke.ca
lab head
Maude Hamilton
contact affiliationDepartment of Immunology and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Canada
contact emailmaude.hamilton@usherbrooke.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
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