PXD038985-2

PXD038985 is an original dataset announced via ProteomeXchange.

Title	Comparison of peptidomes extracted from healthy tissue, tumor tissue of the parotid glands and saliva samples.
Description	Salivary gland tumors account for approximately 3% of head and neck cancers and are extremely diverse in terms of clinical manifestation and histology. In this paper, we present the preliminary results of research aimed at developing a method for identifying neoplastic lesions in the salivary glands based on the analysis of peptides contained in the patients' saliva. The composition of the peptidome extracted from 18 cancer tissue samples and 18 healthy tissues samples were analyzed using mass-spectrometry. PBS and 0.1% trifluoroacetic acid were used to extract the peptide fraction from the tissue samples. The peptidome compositions of the saliva of patients (11 samples) and the saliva of healthy individuals (8 samples) were also analyzed. Using the bioinformatics analysis, a group of peptides (109 peptides) was selected that are present only in extracts from cancer tissues and in samples of patients' saliva. Some of the identified peptides come from proteins that in previous studies were indicated as potential biomarkers of salivary gland tumors (ANXA1, BPIFA2, FGB, GAPDH, HSPB1, IGHG1, VIM) or tumors of other tissues or organs (SERPINA1, APOA2, CSTB, GSTP1, S100A8, S100A9, TPI1). Using the OpenProt database, potential peptide biomarkers derived from pseudogenes or protein isoforms were also identified. Potential peptide biomarkers identified in this paper may be used as a starting point for studies on a larger number of clinical samples aimed at possible selection of a biomarker of clinical significance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:57:56.158.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michał Puchalski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-20 20:03:10	ID requested
1	2024-09-10 07:25:45	announced
⏵ 2	2024-10-22 06:57:56	announced	2024-10-22: Updated project metadata.

10.3390/IJMS25168799;

submitter keyword: cancer, LC-MS, saliva,salivary glands, peptidome, biomarkers

Stanisław Ołdziej
contact affiliation	Laboratory of Biopolymers Structure, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, Poland
contact email	stanislaw.oldziej@biotech.ug.edu.pl
lab head
Michał Puchalski
contact affiliation	Laboratory of Biopolymers Structure, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University Of Gdańsk, Poland
contact email	michal.puchalski@phdstud.ug.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD038985

PRIDE project URI

• PRIDE
  1. PXD038985
     1. Label: PRIDE project
     2. Name: Comparison of peptidomes extracted from healthy tissue, tumor tissue of the parotid glands and saliva samples.