PXD038985 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparison of peptidomes extracted from healthy tissue, tumor tissue of the parotid glands and saliva samples. |
Description | Salivary gland tumors account for approximately 3% of head and neck cancers and are extremely diverse in terms of clinical manifestation and histology. In this paper, we present the preliminary results of research aimed at developing a method for identifying neoplastic lesions in the salivary glands based on the analysis of peptides contained in the patients' saliva. The composition of the peptidome extracted from 18 cancer tissue samples and 18 healthy tissues samples were analyzed using mass-spectrometry. PBS and 0.1% trifluoroacetic acid were used to extract the peptide fraction from the tissue samples. The peptidome compositions of the saliva of patients (11 samples) and the saliva of healthy individuals (8 samples) were also analyzed. Using the bioinformatics analysis, a group of peptides (109 peptides) was selected that are present only in extracts from cancer tissues and in samples of patients' saliva. Some of the identified peptides come from proteins that in previous studies were indicated as potential biomarkers of salivary gland tumors (ANXA1, BPIFA2, FGB, GAPDH, HSPB1, IGHG1, VIM) or tumors of other tissues or organs (SERPINA1, APOA2, CSTB, GSTP1, S100A8, S100A9, TPI1). Using the OpenProt database, potential peptide biomarkers derived from pseudogenes or protein isoforms were also identified. Potential peptide biomarkers identified in this paper may be used as a starting point for studies on a larger number of clinical samples aimed at possible selection of a biomarker of clinical significance. |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-10 |
AnnouncementXML | Submission_2024-09-10_07:25:44.826.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michał Puchalski |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-20 20:03:10 | ID requested | |
⏵ 1 | 2024-09-10 07:25:45 | announced | |
2 | 2024-10-22 06:57:56 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: cancer, LC-MS, saliva,salivary glands, peptidome, biomarkers |
Contact List
Stanisław Ołdziej |
contact affiliation | Laboratory of Biopolymers Structure, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, Poland |
contact email | stanislaw.oldziej@biotech.ug.edu.pl |
lab head | |
Michał Puchalski |
contact affiliation | Laboratory of Biopolymers Structure, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University Of Gdańsk, Poland |
contact email | michal.puchalski@phdstud.ug.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038985
- Label: PRIDE project
- Name: Comparison of peptidomes extracted from healthy tissue, tumor tissue of the parotid glands and saliva samples.