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PXD038826-2

PXD038826 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGrowth of complete ammonia oxidizers on guanidine
DescriptionThe nitrogen rich compound guanidine occurs widely in nature and is used by microbes as a nitrogen source, but microorganisms that grow on guanidine have not yet been discovered. Here we show that complete ammonia-oxidizing microbes (comammox), but no other known nitrifiers, encode homologues of a guanidinase and that the comammox isolate Nitrospira inopinata grows on guanidine as sole source of energy and reductant. Proteomics, kinetic enzyme characterization, and the crystal structure of the N. inopinata guanidinase homologue demonstrated that it is a bona fide guanidinase. Transcription of comammox guanidinases was induced in wastewater treatment plant microbiomes upon incubation with guanidine, and guanidine degradation was detected in these systems. The discovery of guanidine as a selective growth substrate for comammox shows a unique niche of these globally important nitrifiers and offers new options for their isolation as well as for targeted manipulation of nitrifier communities.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:52:26.307.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNico Jehmlich
SpeciesList scientific name: Candidatus Nitrospira inopinata; NCBI TaxID: 1715989;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-13 23:24:31ID requested
12024-08-03 00:05:18announced
22024-10-22 06:52:26announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: nitrogen, proteomics,Guanidine, complete ammonia oxidizers, reductant
Contact List
Nico Jehmlich
contact affiliationHelmholtz-Centre for Environmental Research - UFZ GmbH Department of Molecular Systems Biology Permoserstrasse 15 04318 Leipzig, Germany
contact emailnico.jehmlich@ufz.de
lab head
Nico Jehmlich
contact affiliationHelmholtz-Centre for Environmental Research - UFZ
contact emailnico.jehmlich@ufz.de
dataset submitter
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Dataset FTP location
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