PXD038826-1

PXD038826 is an original dataset announced via ProteomeXchange.

Title	Growth of complete ammonia oxidizers on guanidine
Description	The nitrogen rich compound guanidine occurs widely in nature and is used by microbes as a nitrogen source, but microorganisms that grow on guanidine have not yet been discovered. Here we show that complete ammonia-oxidizing microbes (comammox), but no other known nitrifiers, encode homologues of a guanidinase and that the comammox isolate Nitrospira inopinata grows on guanidine as sole source of energy and reductant. Proteomics, kinetic enzyme characterization, and the crystal structure of the N. inopinata guanidinase homologue demonstrated that it is a bona fide guanidinase. Transcription of comammox guanidinases was induced in wastewater treatment plant microbiomes upon incubation with guanidine, and guanidine degradation was detected in these systems. The discovery of guanidine as a selective growth substrate for comammox shows a unique niche of these globally important nitrifiers and offers new options for their isolation as well as for targeted manipulation of nitrifier communities.
HostingRepository	PRIDE
AnnounceDate	2024-08-03
AnnouncementXML	Submission_2024-08-03_00:05:17.635.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nico Jehmlich
SpeciesList	scientific name: Candidatus Nitrospira inopinata; NCBI TaxID: 1715989;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-13 23:24:31	ID requested
⏵ 1	2024-08-03 00:05:18	announced
2	2024-10-22 06:52:26	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: nitrogen, proteomics,Guanidine, complete ammonia oxidizers, reductant

Nico Jehmlich
contact affiliation	Helmholtz-Centre for Environmental Research - UFZ GmbH Department of Molecular Systems Biology Permoserstrasse 15 04318 Leipzig, Germany
contact email	nico.jehmlich@ufz.de
lab head
Nico Jehmlich
contact affiliation	Helmholtz-Centre for Environmental Research - UFZ
contact email	nico.jehmlich@ufz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038826
     1. Label: PRIDE project
     2. Name: Growth of complete ammonia oxidizers on guanidine