PXD038676-1

PXD038676 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of isolated murine neurons
Description	Muscle isoform of fructose 1,6-bisphosphatase (FBP2) is a regulatory enzyme of glycogen synthesis from non-carbohydrates and a multifunctional protein indispensable for induction and maintenance of the long-term potentiation (the LTP), a molecular/cellular mechanism of memory formation in the hippocampus. During the LTP formation FBP2 affects phosphorylation status of the CAMKIV and CAMKII, protein kinases involved directly and indirectly in regulation of gene transcription in neurons. The results of the study demonstrate the effect of partial FBP2 silencing (using the shRNA technique; three days of the silencing) in 17-day-old culture of mice hippocampal neurons on their proteome. Hippocampal neurons were isolated from newborn BALB/c mice and cultured at 37°C in a humidified atmosphere with 5% CO2 as described previously [Mozrzymas et al.] with a minor modification - the glucose concentration in the culture medium was 2.5 mM. Mozrzymas J., Szczesny T., Rakus, D. The effect of glycogen phosphorolysis on basal glutaminergic transmission. Biochem. Biophys. Res. Commun. 2011, 404, 652–655.
HostingRepository	PRIDE
AnnounceDate	2026-01-24
AnnouncementXML	Submission_2026-01-23_21:56:33.118.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacek Wisniewski
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-07 13:08:58	ID requested
⏵ 1	2026-01-23 21:56:33	announced

Dataset with its publication pending

submitter keyword: Neuron, Glyconeogenesis

Jacek R. Wisniewski
contact affiliation	Max Planck Institute of Biochemitry, Martinsried, Germany
contact email	jwisniew@biochem.mpg.de
lab head
Jacek Wisniewski
contact affiliation	Proteomics
contact email	jwisniew@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD038676

PRIDE project URI

• PRIDE
  1. PXD038676
     1. Label: PRIDE project
     2. Name: Proteomic analysis of isolated murine neurons