Muscle isoform of fructose 1,6-bisphosphatase (FBP2) is a regulatory enzyme of glycogen synthesis from non-carbohydrates and a multifunctional protein indispensable for induction and maintenance of the long-term potentiation (the LTP), a molecular/cellular mechanism of memory formation in the hippocampus. During the LTP formation FBP2 affects phosphorylation status of the CAMKIV and CAMKII, protein kinases involved directly and indirectly in regulation of gene transcription in neurons. The results of the study demonstrate the effect of partial FBP2 silencing (using the shRNA technique; three days of the silencing) in 17-day-old culture of mice hippocampal neurons on their proteome. Hippocampal neurons were isolated from newborn BALB/c mice and cultured at 37°C in a humidified atmosphere with 5% CO2 as described previously [Mozrzymas et al.] with a minor modification - the glucose concentration in the culture medium was 2.5 mM. Mozrzymas J., Szczesny T., Rakus, D. The effect of glycogen phosphorolysis on basal glutaminergic transmission. Biochem. Biophys. Res. Commun. 2011, 404, 652–655.