PXD037690-2

PXD037690 is an original dataset announced via ProteomeXchange.

Title	Epididymal epithelial degeneration and lipid metabolism impairment account for male infertility in occludin knockout mice
Description	Occludin (OCLN) is a tight junction protein and Ocln deletion mutation causes male infertility in mice. However, the role of OCLN in male reproductive system remains unknown. In this study, we found defective proximal epididymis in Ocln-null mice with impaired sperm mobility and fertilization, but no defects in testicular spermatogenesis. Integrative omics analysis revealed the downregulation of gene clusters enriched in acid secretion and fatty acid metabolism in the Ocln-null epididymis, especially the enzymes related to the unsaturated arachidonic acid pathway. The number of proton-pump V-ATPase-expression clear cells, a key player of luminal acidification in the epididymis, declined drastically from prepubertal age before sperm arrival but not in the early postnatal-age. This was accompanied by clear cell apoptosis and increase in pH in the epididymal fluid of OCLN-deficient mice. The lipidomics results showed significantly increased levels of specific DAGs conjugated to unsaturated fatty acids in the Ocln-mutant. Immunofluorescent labeling showed that the arachidonic acid converting enzyme PTGDS and phospholipase PLA2g12a were prominently altered in the principal cells and luminal contents of the Ocln-mutant epididymis. Whereas the carboxylate ester lipase CES1, originally enriched in the WT basal cells, was found upregulated in the Ocln-mutant principal cells. Overall, this study demonstrates that OCLN is essential for maintaining the survival of acid-secreting clear cells and unsaturated fatty acid catabolism in the mouse epididymis, as well as ensuring sperm maturation and male fertility.
HostingRepository	iProX
AnnounceDate	2022-10-25
AnnouncementXML	Submission_2022-12-24_20:58:23.901.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Winnie Shum
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-25 00:16:11	ID requested
1	2022-11-08 00:15:25	announced
⏵ 2	2022-12-24 20:58:24	announced	2022-12-25: Update publication information.

Liu BY, Zhang BL, Gao DY, Li Q, Xu XY, Shum W, Epididymal epithelial degeneration and lipid metabolism impairment account for male infertility in occludin knockout mice. Front Endocrinol (Lausanne), 13():1069319(2022) [pubmed]

submitter keyword: whole epididymis

Winnie Shum
contact affiliation	ShanghaiTech University
contact email	shumw@shanghaitech.edu.cn
lab head
Winnie Shum
contact affiliation	ShanghaiTech University
contact email	shumw@shanghaitech.edu.cn
dataset submitter

iProX dataset URI