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PXD037690-1

PXD037690 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEpididymal epithelial degeneration and lipid metabolism impairment account for male infertility in occludin knockout mice
DescriptionOccludin (OCLN) is a tight junction protein and Ocln deletion mutation causes male infertility in mice. However, the role of OCLN in male reproductive system remains unknown. In this study, we found defective proximal epididymis in Ocln-null mice with impaired sperm mobility and fertilization, but no defects in testicular spermatogenesis. Integrative omics analysis revealed the downregulation of gene clusters enriched in acid secretion and fatty acid metabolism in the Ocln-null epididymis, especially the enzymes related to the unsaturated arachidonic acid pathway. The number of proton-pump V-ATPase-expression clear cells, a key player of luminal acidification in the epididymis, declined drastically from prepubertal age before sperm arrival but not in the early postnatal-age. This was accompanied by clear cell apoptosis and increase in pH in the epididymal fluid of OCLN-deficient mice. The lipidomics results showed significantly increased levels of specific DAGs conjugated to unsaturated fatty acids in the Ocln-mutant. Immunofluorescent labeling showed that the arachidonic acid converting enzyme PTGDS and phospholipase PLA2g12a were prominently altered in the principal cells and luminal contents of the Ocln-mutant epididymis. Whereas the carboxylate ester lipase CES1, originally enriched in the WT basal cells, was found upregulated in the Ocln-mutant principal cells. Overall, this study demonstrates that OCLN is essential for maintaining the survival of acid-secreting clear cells and unsaturated fatty acid catabolism in the mouse epididymis, as well as ensuring sperm maturation and male fertility.
HostingRepositoryiProX
AnnounceDate2022-10-25
AnnouncementXMLSubmission_2022-11-08_00:15:25.207.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWinnie Shum
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-25 00:16:11ID requested
12022-11-08 00:15:25announced
22022-12-24 20:58:24announced2022-12-25: Update publication information.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: whole epididymis
Contact List
Winnie Shum
contact affiliationShanghaiTech University
contact emailshumw@shanghaitech.edu.cn
lab head
Winnie Shum
contact affiliationShanghaiTech University
contact emailshumw@shanghaitech.edu.cn
dataset submitter
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