PXD037510-3

PXD037510 is an original dataset announced via ProteomeXchange.

Title	Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover
Description	Protein N-terminal (Nt) acetylation is one of the most abundant modifications in eukaryotes, covering ~50-80 % of the proteome, depending on species. Cells with defective Nt-acetylation display a wide array of phenotypes such as impaired growth, mating defects and increased stress sensitivity. However, the pleiotropic nature of these effects has hampered our understanding of the functional impact of protein Nt-acetylation. The main enzyme responsible for Nt-acetylation throughout the eukaryotic kingdom is the N-terminal acetyltransferase NatA. Here we employed a multi-dimensional proteomics approach to analyze Saccharomyces cerevisiae lacking NatA activity, which caused global proteome remodeling. Pulsed-SILAC experiments revealed that NatA-deficient strains consistently increased degradation of ribosomal proteins compared to wild type. Explaining this phenomenon, thermal proteome profiling uncovered decreased thermostability of ribosomes in NatA-knockouts. Our data are in agreement with a role for Nt-acetylation in promoting stability for parts of the proteome by enhancing the avidity of protein-protein interactions and folding.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:15.541.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ulises H Guzman
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	ubiquitination signature dipeptidyl lysine; acetylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-17 03:26:00	ID requested
1	2023-10-24 19:22:59	announced
2	2023-11-14 09:12:44	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:13:19	announced	2024-10-22: Updated project metadata.

Guzman UH, Aksnes H, Ree R, Krogh N, Jakobsson ME, Jensen LJ, Arnesen T, Olsen JV, Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover in Saccharomyces cerevisiae. Nat Commun, 14(1):4517(2023) [pubmed]

10.1038/s41467-023-40224-x;

submitter keyword: Sacharomyces cerevisiae , TPP,Nt-acetylation, PSilac

Jesper V. Olsen
contact affiliation	Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
contact email	jesper.olsen@cpr.ku.dk
lab head
Ulises H Guzman
contact affiliation	Novo Nordisk Foundation Center for Protein Research
contact email	ulysseshrdz@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037510
     1. Label: PRIDE project
     2. Name: Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover