PXD037510 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover |
Description | Protein N-terminal (Nt) acetylation is one of the most abundant modifications in eukaryotes, covering ~50-80 % of the proteome, depending on species. Cells with defective Nt-acetylation display a wide array of phenotypes such as impaired growth, mating defects and increased stress sensitivity. However, the pleiotropic nature of these effects has hampered our understanding of the functional impact of protein Nt-acetylation. The main enzyme responsible for Nt-acetylation throughout the eukaryotic kingdom is the N-terminal acetyltransferase NatA. Here we employed a multi-dimensional proteomics approach to analyze Saccharomyces cerevisiae lacking NatA activity, which caused global proteome remodeling. Pulsed-SILAC experiments revealed that NatA-deficient strains consistently increased degradation of ribosomal proteins compared to wild type. Explaining this phenomenon, thermal proteome profiling uncovered decreased thermostability of ribosomes in NatA-knockouts. Our data are in agreement with a role for Nt-acetylation in promoting stability for parts of the proteome by enhancing the avidity of protein-protein interactions and folding. |
HostingRepository | PRIDE |
AnnounceDate | 2023-10-25 |
AnnouncementXML | Submission_2023-10-24_19:22:58.798.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | UlisesH Guzman |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | ubiquitination signature dipeptidyl lysine; acetylated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-17 03:26:00 | ID requested | |
⏵ 1 | 2023-10-24 19:22:59 | announced | |
2 | 2023-11-14 09:12:44 | announced | 2023-11-14: Updated project metadata. |
Publication List
Guzman UH, Aksnes H, Ree R, Krogh N, Jakobsson ME, Jensen LJ, Arnesen T, Olsen JV, Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover in Saccharomyces cerevisiae. Nat Commun, 14(1):4517(2023) [pubmed] |
Keyword List
submitter keyword: Sacharomyces cerevisiae , TPP,Nt-acetylation, PSilac |
Contact List
Jesper V.Olsen |
contact affiliation | Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark |
contact email | jesper.olsen@cpr.ku.dk |
lab head | |
UlisesH Guzman |
contact affiliation | Novo Nordisk Foundation Center for Protein Research |
contact email | ulysseshrdz@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037510
- Label: PRIDE project
- Name: Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover