PXD037285-2

PXD037285 is an original dataset announced via ProteomeXchange.

Title	Decrypting drug actions and protein modifications by dose- and time-resolved chemical proteomics
Description	Measuring dose-dependent effects of drugs on post-translational modifications on a proteome-wide scale reveals how these drugs work in cells. Here, we present a quantitative chemical proteomic approach termed decryptM, able to assess target and pathway engagement as well as the mode of action (MoA) of diverse cancer drugs in cells by measuring their dose- (and time-) resolved modulation of PTMs on a proteomic scale. Data collected for 31 drugs, representing six drug classes in 13 human cell lines, demonstrate that the approach is widely applicable. The body of data represents ~1.8 million quantitative cellular drug assays (dose-response curves) including 47502 regulated p-peptides (of 124660 detected on 11982 proteins), 7316 Ubi-peptides (of 9173 detected on 3006 proteins), and 546 Ac-peptides (of 2478 detected on 1377 proteins). Most PTMs were not regulated by most drugs, which is highly valuable information for understanding which pathways are addressed (or not) by each drug in cells. The decryptM data have been incorporated into ProteomicsDB and can be explored interactively. The raw files, searches, curves files, and result PDFs can be downloaded here. For details, have a look at the Experiment_summary.xlsx. Paper: doi/10.1126/science.ade3925
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:12.588.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian P Bayer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine; phosphorylated residue; acetylated residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-11 01:59:44	ID requested
1	2023-03-18 00:39:44	announced
⏵ 2	2023-11-14 08:59:13	announced	2023-11-14: Updated project metadata.
3	2024-04-02 06:40:37	announced	2024-04-02: Updated project metadata.

Dataset with its publication pending

submitter keyword: KAT, screen, drug mode of action, post-translational modifciation, time-resolved, inhibitors,decryptM, proteome-wide, HDAC, dose-resolved, Kinase

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich
contact email	kuster@tum.de
lab head
Florian P Bayer
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich
contact email	f.bayer@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD037285

PRIDE project URI

• PRIDE
  1. PXD037285
     1. Label: PRIDE project
     2. Name: Decrypting drug actions and protein modifications by dose- and time-resolved chemical proteomics