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PXD037285 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDecrypting drug actions and protein modifications by dose- and time-resolved chemical proteomics
DescriptionMeasuring dose-dependent effects of drugs on post-translational modifications on a proteome-wide scale reveals how these drugs work in cells. Here, we present a quantitative chemical proteomic approach termed decryptM, able to assess target and pathway engagement as well as the mode of action (MoA) of diverse cancer drugs in cells by measuring their dose- (and time-) resolved modulation of PTMs on a proteomic scale. Data collected for 31 drugs, representing six drug classes in 13 human cell lines, demonstrate that the approach is widely applicable. The body of data represents ~1.8 million quantitative cellular drug assays (dose-response curves) including 47502 regulated p-peptides (of 124660 detected on 11982 proteins), 7316 Ubi-peptides (of 9173 detected on 3006 proteins), and 546 Ac-peptides (of 2478 detected on 1377 proteins). Most PTMs were not regulated by most drugs, which is highly valuable information for understanding which pathways are addressed (or not) by each drug in cells. The decryptM data have been incorporated into ProteomicsDB and can be explored interactively. The raw files, searches, curves files, and result PDFs can be downloaded here. For details, have a look at the Experiment_summary.xlsx. Paper: doi/10.1126/science.ade3925
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFlorian PBayer
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitination signature dipeptidyl lysine; phosphorylated residue; acetylated residue
InstrumentQ Exactive HF; Orbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-11 01:59:44ID requested
12023-03-18 00:39:44announced
22023-11-14 08:59:13announced2023-11-14: Updated project metadata.
32024-04-02 06:40:37announced2024-04-02: Updated project metadata.
Publication List
Keyword List
submitter keyword: KAT, screen, drug mode of action, post-translational modifciation, time-resolved, inhibitors,decryptM, proteome-wide, HDAC, dose-resolved, Kinase
Contact List
contact affiliationChair of Proteomics and Bioanalytics, Technical University of Munich
contact emailkuster@tum.de
lab head
Florian PBayer
contact affiliationChair of Proteomics and Bioanalytics, Technical University of Munich
contact emailf.bayer@tum.de
dataset submitter
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Dataset FTP location
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