PXD037193 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chronic exposure to multiple stressors alters the salivary proteome of piglets |
Description | Monitoring chronic stress in pigs is not only essential in view of animal welfare but is also important for the farmer given stress influences the zootechnical performance of the pigs and increases their susceptibility to infectious diseases. Saliva analysis could be a non-invasive, objective evaluation tool to asses stress levels. To investigate the use of saliva as a stress monitoring tool, twenty-four 4-day-old piglets were transferred to artificail brooders. At the age of 7 days, they were assigend to either the control or the stressed group and reared for three weeks. Piglets in the stressed group were exposed to overcrowding, absence of cage enrichment, and frequent mixing between pens. Shotgun analysis using an isobaric labelling method (iTRAQ) for tandem mass spectrometry performed on saliva samples taken after three weeks of chronic stress identified 392 proteins of which 20 proteins displayed siginifcantly altered concentrations. From these 20 proteins, eight were selected for further validation using parallel reaction monitoring (PRM). For this validation, additional saliva samples were taken after one week, and the samples that were taken three weeks after the start of the experiment were analysed to verify the profile over time. This targeted PRM analysis confiremd that alpha-2-HS-glycoprotein was upregulated in the stressed group after one and three weeks while odorant-binding protein, chitinase, long palate lung and nasal epithelium protein 5, lipocalin-1, and vomeromodulin-like protein were present in lower concentrations in saliva of the stressed pigs, albeit only after three weeks. These results indicate that the porcine salivary proteome is altered by chronic exposure to multiple stressors. The affected proteins could be used as salivary biomarkers to identify welfare problems at the farm and facilitate research to optimise rearing conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:00:55.866.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD037193 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Sara Prims |
SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-05 01:22:08 | ID requested | |
1 | 2023-06-12 04:12:01 | announced | |
⏵ 2 | 2023-11-14 09:00:56 | announced | 2023-11-14: Updated project metadata. |
3 | 2024-10-22 05:47:42 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: PRM, stress, saliva, iTRAQ,Pig |
Contact List
Chris Van Ginneken |
contact affiliation | Department of Veterinary Sciences Comparative Perinatal Development (CoPeD) University of Antwerp Belgium |
contact email | chris.vanginneken@uantwerpen.be |
lab head | |
Sara Prims |
contact affiliation | UAntwerpen |
contact email | sara.prims@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037193
- Label: PRIDE project
- Name: Chronic exposure to multiple stressors alters the salivary proteome of piglets