PXD037193-1

PXD037193 is an original dataset announced via ProteomeXchange.

Title	Chronic exposure to multiple stressors alters the salivary proteome of piglets
Description	Monitoring chronic stress in pigs is not only essential in view of animal welfare but is also important for the farmer given stress influences the zootechnical performance of the pigs and increases their susceptibility to infectious diseases. Saliva analysis could be a non-invasive, objective evaluation tool to asses stress levels. To investigate the use of saliva as a stress monitoring tool, twenty-four 4-day-old piglets were transferred to artificail brooders. At the age of 7 days, they were assigend to either the control or the stressed group and reared for three weeks. Piglets in the stressed group were exposed to overcrowding, absence of cage enrichment, and frequent mixing between pens. Shotgun analysis using an isobaric labelling method (iTRAQ) for tandem mass spectrometry performed on saliva samples taken after three weeks of chronic stress identified 392 proteins of which 20 proteins displayed siginifcantly altered concentrations. From these 20 proteins, eight were selected for further validation using parallel reaction monitoring (PRM). For this validation, additional saliva samples were taken after one week, and the samples that were taken three weeks after the start of the experiment were analysed to verify the profile over time. This targeted PRM analysis confiremd that alpha-2-HS-glycoprotein was upregulated in the stressed group after one and three weeks while odorant-binding protein, chitinase, long palate lung and nasal epithelium protein 5, lipocalin-1, and vomeromodulin-like protein were present in lower concentrations in saliva of the stressed pigs, albeit only after three weeks. These results indicate that the porcine salivary proteome is altered by chronic exposure to multiple stressors. The affected proteins could be used as salivary biomarkers to identify welfare problems at the farm and facilitate research to optimise rearing conditions.
HostingRepository	PRIDE
AnnounceDate	2023-06-12
AnnouncementXML	Submission_2023-06-12_04:12:00.922.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037193
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	SaraPrims
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-05 01:22:08	ID requested
⏵ 1	2023-06-12 04:12:01	announced
2	2023-11-14 09:00:56	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:47:42	announced	2024-10-22: Updated project metadata.

10.6019/PXD037193;

submitter keyword: PRM, stress, saliva, iTRAQ,Pig

Chris VanGinneken
contact affiliation	Department of Veterinary Sciences Comparative Perinatal Development (CoPeD) University of Antwerp Belgium
contact email	chris.vanginneken@uantwerpen.be
lab head
SaraPrims
contact affiliation	UAntwerpen
contact email	sara.prims@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD037193

PRIDE project URI

• PRIDE
  1. PXD037193
     1. Label: PRIDE project
     2. Name: Chronic exposure to multiple stressors alters the salivary proteome of piglets