PXD036930-2

PXD036930 is an original dataset announced via ProteomeXchange.

Title	mass spectrometry analysis of in vitro kinase assay for DNA-PKcs and TAF7
Description	To examine whether TAF7 could be phosphorylated by DNA-PKcs directly. An in vitro kinase assays were performed. Firstly, human DNA-PKcs was purified from TGF1 treated HK2 cells by immunoprecipitation with a DNA-PKcs antibody (abcam) and IgG was used as a negative control. The purified proteins and human GST-TAF7 (purchased from proteintech) were incubated in kinase assay buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM MgCl2, and 1 mM MnCl2) supplemented with 200 M ATP in 50 l reactions for 30 min at 30°C. Then, phosphorylation of TAF7 was analyzed by mass spectrometry (MS)
HostingRepository	iProX
AnnounceDate	2022-09-22
AnnouncementXML	Submission_2023-03-28_20:34:55.508.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yunwen Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-21 22:46:17	ID requested
1	2022-09-21 22:46:28	announced
⏵ 2	2023-03-28 20:34:56	announced	2023-03-29: Update publication information.

Yang Y, Liu S, Wang P, Ouyang J, Zhou N, Zhang Y, Huang S, Jia Z, Zhang A, DNA-dependent protein kinase catalytic subunit (DNA-PKcs) drives chronic kidney disease progression in male mice. Nat Commun, 14(1):1334(2023) [pubmed]

submitter keyword: in vitro kinase, DNA-PKcs, TAF7

Zhanjun Jia
contact affiliation	Department of Nephrology, Children’s Hospital of Nanjing Medical University
contact email	jiazj72@hotmail.com
lab head
Yunwen Yang
contact affiliation	Children's Hospital of Nanjing Medical University
contact email	YYW09030126@163.com
dataset submitter

iProX dataset URI