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PXD036930-1

PXD036930 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	mass spectrometry analysis of in vitro kinase assay for DNA-PKcs and TAF7
Description	To examine whether TAF7 could be phosphorylated by DNA-PKcs directly. An in vitro kinase assays were performed. Firstly, human DNA-PKcs was purified from TGF1 treated HK2 cells by immunoprecipitation with a DNA-PKcs antibody (abcam) and IgG was used as a negative control. The purified proteins and human GST-TAF7 (purchased from proteintech) were incubated in kinase assay buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM MgCl2, and 1 mM MnCl2) supplemented with 200 M ATP in 50 l reactions for 30 min at 30°C. Then, phosphorylation of TAF7 was analyzed by mass spectrometry (MS)
HostingRepository	iProX
AnnounceDate	2022-09-22
AnnouncementXML	Submission_2022-09-21_22:46:27.620.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yunwen Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-21 22:46:17	ID requested
⏵ 1	2022-09-21 22:46:28	announced
2	2023-03-28 20:34:56	announced	2023-03-29: Update publication information.

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: in vitro kinase, DNA-PKcs, TAF7

Contact List

Zhanjun Jia
contact affiliation	Department of Nephrology, Children’s Hospital of Nanjing Medical University
contact email	jiazj72@hotmail.com
lab head
Yunwen Yang
contact affiliation	Children's Hospital of Nanjing Medical University
contact email	YYW09030126@163.com
dataset submitter

Full Dataset Link List

iProX dataset URI