PXD035230-2

PXD035230 is an original dataset announced via ProteomeXchange.

Title	Repurposing of a known antipsychotic drug to target arthritogenic synovial fibroblasts
Description	We applied quantitative mass spectrometry (MS)-based proteomics to investigate the phosphoproteome of hTNF-stimulated and Amisulpride-treated synovial fibroblasts (SFs), as part of a study aiming to find new potent orally-administered therapeutics to reverse the pathogenic expression signature of arthritogenic SFs. Phosphoproteomics analysis were done by DIA-based phosphoproteomic profiling of synovial fibroblasts. Samples consisted of hTNF-induced WT SFs. Three different time points (5’, 15’, 30’) of hTNF induction were used and compared to cells pretreated with Amisulpride for 1h before being stimulated at similar time points with hTNF (5’, 15’, 30’).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:34.485.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna-Kathrine Pedersen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-11 08:07:04	ID requested
1	2023-04-11 07:13:52	announced
⏵ 2	2023-11-14 08:57:35	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: LC-MSMS, small molecule repurposing, tumor necrosis factor alpha,Rheumatoid arthritis, synovial fibroblasts

Jesper Velgaard Olsen
contact affiliation	Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DK-2200, Denmark
contact email	jesper.olsen@cpr.ku.dk
lab head
Anna-Kathrine Pedersen
contact affiliation	NNF Center for Protein Research, University of Copenhagen
contact email	anna-kathrine.pedersen@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD035230

PRIDE project URI

• PRIDE
  1. PXD035230
     1. Label: PRIDE project
     2. Name: Repurposing of a known antipsychotic drug to target arthritogenic synovial fibroblasts