PXD033942-2

PXD033942 is an original dataset announced via ProteomeXchange.

Title	Identification of myeloid-derived growth factor as a mechanically-induced, growth-promoting angiocrine signal for human hepatocytes
Description	Recently, we have shown that after partial hepatectomy (PHx), an increased hepatic blood flow initiates liver growth in mice by vasodilation and mechanically-triggered release of angiocrine signals. Here, we use mass spectrometry to identify a mechanically-induced angiocrine signal in human hepatic endothelial cells, that is, myeloid-derived growth factor (MYDGF). We show that it induces proliferation and promotes survival of primary human hepatocytes derived from different donors in two-dimensional cell culture, via activation of mitogen-activated protein kinase (MAPK) and signal transducer and activator of transcription 3 (STAT3). MYDGF also enhances proliferation of human hepatocytes in three-dimensional organoids. In vivo, genetic deletion of MYDGF decreases hepatocyte proliferation in the regenerating mouse liver after PHx; conversely, adeno-associated viral delivery of MYDGF increases hepatocyte proliferation and MAPK signaling after PHx. We conclude that MYDGF represents a mechanically-induced angiocrine signal and that it triggers growth of, and provides protection to, primary mouse and human hepatocytes
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:18:47.282.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paula Follert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-17 08:29:04	ID requested
1	2023-12-28 11:09:01	announced
⏵ 2	2024-10-22 06:18:47	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, LC-MS-MS, Myeloid-derived growth factor, Liver

Eckhard Lammert
contact affiliation	Institute of Metabolic Physiology Heinrich Heine University of Duesseldorf Universitätsstr. 1 D-40225 Duesseldorf Germany
contact email	lammert@hhu.de
lab head
Paula Follert
contact affiliation	Heinrich Heine University Institute for metabolic physiology
contact email	Paula.Follert@hhu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033942
     1. Label: PRIDE project
     2. Name: Identification of myeloid-derived growth factor as a mechanically-induced, growth-promoting angiocrine signal for human hepatocytes