PXD033942 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of myeloid-derived growth factor as a mechanically-induced, growth-promoting angiocrine signal for human hepatocytes |
Description | Recently, we have shown that after partial hepatectomy (PHx), an increased hepatic blood flow initiates liver growth in mice by vasodilation and mechanically-triggered release of angiocrine signals. Here, we use mass spectrometry to identify a mechanically-induced angiocrine signal in human hepatic endothelial cells, that is, myeloid-derived growth factor (MYDGF). We show that it induces proliferation and promotes survival of primary human hepatocytes derived from different donors in two-dimensional cell culture, via activation of mitogen-activated protein kinase (MAPK) and signal transducer and activator of transcription 3 (STAT3). MYDGF also enhances proliferation of human hepatocytes in three-dimensional organoids. In vivo, genetic deletion of MYDGF decreases hepatocyte proliferation in the regenerating mouse liver after PHx; conversely, adeno-associated viral delivery of MYDGF increases hepatocyte proliferation and MAPK signaling after PHx. We conclude that MYDGF represents a mechanically-induced angiocrine signal and that it triggers growth of, and provides protection to, primary mouse and human hepatocytes |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-28 |
AnnouncementXML | Submission_2023-12-28_11:09:01.469.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Paula Follert |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-17 08:29:04 | ID requested | |
⏵ 1 | 2023-12-28 11:09:01 | announced | |
2 | 2024-10-22 06:18:47 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, LC-MS-MS, Myeloid-derived growth factor, Liver |
Contact List
Eckhard Lammert |
contact affiliation | Institute of Metabolic Physiology Heinrich Heine University of Duesseldorf Universitätsstr. 1 D-40225 Duesseldorf Germany |
contact email | lammert@hhu.de |
lab head | |
Paula Follert |
contact affiliation | Heinrich Heine University Institute for metabolic physiology |
contact email | Paula.Follert@hhu.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033942
- Label: PRIDE project
- Name: Identification of myeloid-derived growth factor as a mechanically-induced, growth-promoting angiocrine signal for human hepatocytes