PXD033034-1

PXD033034 is an original dataset announced via ProteomeXchange.

Title	Large-scale multi-omic analysis reveals neurosyphilis diagnostic biomarkers and pathogenesis
Description	Neurosyphilis (NS) is a central nervous system infection caused by Treponema pallidum subspecies pallidum. NS has been associated with asymptomatic meningitis, which promotes severe and irreversible organ dysfunction and damage. Therefore, investigating NS pathogenesis is of great importance as this will likely reduce the incidence of sequelae. Besides, diagnosis of NS is quite challenging for clinicians due to lack of gold standard tests for diagnosing NS modalities. Here, using proteomic and machine learning model techniques, we characterize 223 cerebrospinal fluid (CSF) to identify the diagnostic markers of NS and provide mechanistic insights on associated inflammatory responses. Three biomarkers were of diagnostic value for NS through multicenter verification of additional 115 CSF samples. Through proteomics and single cell transcriptome analysis of brain tissue and CSF from NS patients, combined with brain organoid model and histopathological analysis, we found that inflammatory injury in syphilis is predominantly mediated through the Toll-like/NF-κB signal pathway. We anticipate that the biomarkers identified will become an effective means for assisting diagnosis of NS in the future. Our insights into the NS pathogenesis in brain tissue can be useful for discovering therapeutics or benefit drug target discovery for NS patients.
HostingRepository	iProX
AnnounceDate	2022-04-07
AnnouncementXML	Submission_2024-02-17_22:37:35.085.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jie Ma
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-06 20:30:12	ID requested
⏵ 1	2024-02-17 22:37:35	announced
2	2024-02-17 22:46:29	announced	2024-02-18: Update information.

Dataset with its publication pending

submitter keyword: Neurosyphilis, multi-omic, biomarker

Ling Leng
contact affiliation	Peking Union Medical College Hospital
contact email	lengling@pumch.cn
lab head
Jie Ma
contact affiliation	National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics
contact email	majie@ncpsb.org.cn
dataset submitter

iProX dataset URI